Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8392, USA.
Anal Bioanal Chem. 2010 Jul;397(5):1779-85. doi: 10.1007/s00216-010-3710-z.
The definitive method (DM), now known as the reference measurement procedure (RMP), for the analysis of glucose in serum was originally published in 1982 by the National Institute of Standards and Technology (NIST). Over the years the method has been subject to a number of modifications to adapt to newer technologies and simplify sample preparation. We discuss here an adaptation of the method associated with serum glucose measurements using a modified isotope dilution gas chromatography/mass spectrometry (ID-GC/MS) method. NIST has used this modified method to certify the concentrations of glucose in SRM 965b, Glucose in Frozen Human Serum, and SRM 1950, Metabolites in Human Plasma. Comparison of results from the revised method with certified values for existing Standard Reference Materials (SRMs) demonstrated that these modifications have not affected the quality of the measurements, giving both good precision and accuracy, while reducing the sample preparation time by a day and a half.
血清葡萄糖分析的决定性方法（DM），现在称为参考测量程序（RMP），最初由国家标准与技术研究院（NIST）于 1982 年公布。多年来，该方法经过多次修改，以适应新技术并简化样品制备。我们在这里讨论一种与使用改良的同位素稀释气相色谱/质谱（ID-GC/MS）方法测量血清葡萄糖相关的方法改编。NIST 已使用此改良方法对 SRM 965b、冷冻人血清中的葡萄糖和 SRM 1950、人血浆中的代谢物进行了葡萄糖浓度的认证。与现有标准参考物质（SRM）的认证值相比，修订后方法的结果表明，这些修改并未影响测量质量，既具有良好的精密度和准确性，又将样品制备时间缩短了一天半。
