Cardiovascular Medical Research Center, College of Korean Medicine, Dongguk University, Gyeong-Ju, South Korea.
Immunopharmacol Immunotoxicol. 2011 Mar;33(1):201-4. doi: 10.3109/08923973.2010.495393. Epub 2010 Jun 23.
The leaves of Zingiber officinale Roscoe were extracted and the major essential oil composition and immunotoxicity effects were studied. The analyses were conducted by gas chromatography and mass spectroscopy (GC-MS) revealed that the essential oils of Z. officinale leaves. The Z. officinale essential oil yield was 0.26%, and GC/MS analysis revealed that its major constituents were Camphene (5.26%), Phellandrene (6.58%), Zingiberene (36.48%), Geranial (4.32%), β-gurjunene (2.74%), and Citronellol β-sesguiphellandrene (12.31%). The essential oil had a significant toxic effect against early fourth-stage larvae of Aedes aegypti L with an LC(50) value of 46.38 ppm and an LC(90) value of 84.32 ppm. Also, Camphene (≥95.0%), Phellandrene (≥95.0%), Zingiberene (≥95.0%), Geranial (≥95.0%), β-gurjunene (≥97.0%), and Citronellol (≥95.0%) were tested against the F21 laboratory strain of A. aegypti. Zingiberene (≥95.0%) and Citronellol (≥95.0%) have medium activity with an LC(50) value of 99.55 ppm and 141.45 ppm. This indicates that other major compounds may play a more important role in the toxicity of essential oil.
生姜叶被提取出来，并研究了其主要精油成分和免疫毒性作用。通过气相色谱和质谱分析（GC-MS）进行分析，结果表明生姜叶中的精油。生姜精油得率为 0.26%，GC/MS 分析表明其主要成分为莰烯（5.26%）、蒈烯（6.58%）、姜烯（36.48%）、牻牛儿醛（4.32%）、β-古巴烯（2.74%）和β-香叶基倍半水芹烯（12.31%）。该精油对埃及伊蚊四龄早期幼虫具有显著的毒性作用，LC50 值为 46.38ppm，LC90 值为 84.32ppm。此外，莰烯（≥95.0%）、蒈烯（≥95.0%）、姜烯（≥95.0%）、牻牛儿醛（≥95.0%）、β-古巴烯（≥97.0%）和香茅醇（≥95.0%）被测试对埃及伊蚊 F21 实验室品系的活性。姜烯（≥95.0%）和香茅醇（≥95.0%）具有中等活性，LC50 值分别为 99.55ppm 和 141.45ppm。这表明其他主要化合物可能在精油的毒性中发挥更重要的作用。
