Key Laboratory for Agricultural Biodiversity and Pest Management of Ministry of Education, Plant Protection College, Yunnan Agricultural University, Kunming 650201, China.
J Insect Sci. 2010;10:52. doi: 10.1673/031.010.5201.
The white backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae), is a serious pest of rice in Asia. In the present study, inter-simple sequence repeat (ISSR) markers were employed to investigate the genetic diversity and differentiation of 47 populations sampled from 14 prefectures of the Greater Mekong Subregion. A total of 14 selected primers yielded 121 bright and discernible bands, with an average of 8.6 bands per primer. According to the hierarchical analysis of molecular variance (AMOVA), the genetic variation among geographic regions (79.84%) was higher than that of among populations within region (20.16%), and the FST value was 0.72, indicating a high level of genetic differentiation. Neighbor-Joining cluster analysis of the 47 populations showed two major clusters, one consisting of mostly southwestern Yunnan Province and Myanmar populations; and the other one consisting of southeastern and central of Yunnan Province plus Vietnam and Laos populations. No significant positive correlation was observed between genetic and geographic distances by Mantel test (r = 0.2230, p = 0.8448), indicating the role of geographic isolation did not shape the genetic structure of the sampled S. furcifera populations. This paper provides useful data for understanding and speculating the migration of S. furcifera and reveals available information to develop sustainable strategies for manage this long-range migratory pest.
白背飞虱，褐飞虱（半翅目：飞虱科），是亚洲水稻的一种严重害虫。本研究采用简单重复序列间扩增（ISSR）标记对来自大湄公河次区域 14 个省的 47 个种群进行遗传多样性和分化研究。共选用 14 个引物，得到 121 个清晰可辨的条带，平均每个引物 8.6 个条带。基于分子方差分析（AMOVA）的层次分析表明，地理区域间的遗传变异（79.84%）高于区域内种群间的遗传变异（20.16%），FST 值为 0.72，表明遗传分化程度较高。47 个种群的邻接聚类分析显示，两个主要聚类群，一个聚类群主要由云南省西南部和缅甸的种群组成；另一个聚类群主要由云南省东南部和中部以及越南和老挝的种群组成。Mantel 检验显示遗传距离与地理距离之间没有显著的正相关关系（r=0.2230，p=0.8448），表明地理隔离在塑造所研究的褐飞虱种群的遗传结构方面不起作用。本文为了解和推测褐飞虱的迁移提供了有用的数据，并为制定可持续管理这种长距离迁飞害虫的策略提供了可用信息。
