Xie Jia-Nan, Guo Jian-Jun, Jin Dao-Chao, Wang Xue-Jian
The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang, Guizhou 550025, China.
The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang, Guizhou 550025, China
J Insect Sci. 2014 Jan 1;14. doi: 10.1093/jisesa/ieu095. Print 2014.
The white-backed planthopper (WBPH), Sogatella furcifera (Horváth) is a serious pest causing grievous damage to rice plants. In the present study, inter-simple sequence repeats were employed to investigate the genetic diversity of 108 samples from 27 WBPH geographic populations in China. Ten primers were screened out with 147 amplified bands, average percentage of polymorphic bands, polymorphic information content, and marker index were 78.9, 0.456, and 6.753% respectively. The results indicated that genetic diversity was different among populations, but genetic variation was as low as 0.2% among the populations and as high as 99.8% within the same geographic population. Among the examined WBPH populations, genetic distances were weakly correlated to geographic distance, and there was no correlation between genetic identity and elevation. Cluster analysis showed that the 27 WBPH populations studied could be lumped into four clusters, with which the results of principal coordinate analysis (were almost consistent. In conclusion, the molecular genetic data demonstrated that the region consisting of Yunnan, Guizhou, Guangdong, and Guangxi was the first landing area of WBPH in its migrating process from overwintering sites to China.
白背飞虱（Sogatella furcifera (Horváth)）是一种严重危害水稻植株的害虫。在本研究中，利用简单重复序列区间对来自中国27个白背飞虱地理种群的108个样本进行了遗传多样性研究。筛选出10条引物，共扩增出147条带，多态性条带平均百分比、多态信息含量和标记指数分别为78.9%、0.456和6.753%。结果表明，不同种群间遗传多样性存在差异，但种群间遗传变异低至0.2%，同一地理种群内遗传变异高达99.8%。在所检测的白背飞虱种群中，遗传距离与地理距离呈弱相关，遗传同一性与海拔之间无相关性。聚类分析表明，所研究的27个白背飞虱种群可分为4个类群，主坐标分析结果与之基本一致。综上所述，分子遗传数据表明，云南、贵州、广东和广西组成的区域是白背飞虱从越冬地向中国迁飞过程中的首个着陆区。
