Dave B J, Trivedi A H, Adhvaryu S G
Cell Biology Division, Gujarat Cancer and Research Institute, Ahmedabad, India.
Mutagenesis. 1991 Mar;6(2):159-63. doi: 10.1093/mutage/6.2.159.
Pan masala (PM), a dried powdered mixture containing ingredients like areca nut, catechu, lime, cardamom and flavouring agents, is consumed abundantly by Indians and is also exported to Western countries. Pan masala with tobacco (PM-T) is also available on the market. In view of the role of the ingredients of PM in the causation of oral diseases, the possible harmful effects of consuming this complex mixture were analysed in individuals regularly consuming PM and among healthy non-consuming controls without any habit. Three cytogenetic endpoints and two tissues were employed to assess possible DNA damage. Sister chromatid exchange and chromosome aberrations were estimated in the peripheral blood lymphocytes, tissues indirectly exposed to the substance and the frequency of micronucleated cells was scored in the tissue directly in contact with PM, i.e. the exfoliated buccal mucosa cells. All three cytogenetic endpoints demonstrated a statistically significant increase (P less than 0.001) among the PM consumers as compared with the non-consuming controls.
嚼烟（PM）是一种含有槟榔、儿茶、石灰、豆蔻和调味剂等成分的干粉状混合物，印度人大量食用，还出口到西方国家。市场上也有含烟草的嚼烟（PM-T）。鉴于PM成分在口腔疾病病因中的作用，对经常食用PM的个体以及无任何习惯的健康非食用对照者分析了食用这种复合混合物可能产生的有害影响。采用三个细胞遗传学终点和两种组织来评估可能的DNA损伤。在间接接触该物质的外周血淋巴细胞和组织中估计姐妹染色单体交换和染色体畸变，并在与PM直接接触的组织即脱落的颊黏膜细胞中对微核细胞频率进行评分。与非食用对照者相比，所有三个细胞遗传学终点在PM消费者中均显示出统计学上的显著增加（P小于0.001）。
