Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, USA.
Mol Plant Pathol. 2002 May 1;3(3):173-6. doi: 10.1046/j.1364-3703.2002.00108.x.
Summary The rice blast fungus Magnaporthe grisea causes one of the most destructive diseases of rice. Genetic studies of this important pathogen during the past decade have made it an excellent system for investigating fungal-plant interactions. Recently, the well coordinated efforts by the rice blast community have also made significant progresses in genomics studies of M. grisea. BAC contigs were assembled and integrated with the high density genetic map, and 12 674 BAC-ends were sequenced as genome survey sequences. Over ten BAC clones have been sequenced by the shot-gun approach. Preliminary analysis with one completely sequenced BAC clone indicated that M. grisea may have a gene density of 4.2 kb/gene and contain approximately 9000 genes. In addition, > 10 000 ESTs have been sequenced from several cDNA libraries representing different stages of fungal growth, differentiation and plant infection. Currently, there are ongoing projects to sequence additional ESTs, telomeres, and 6X coverage of the M. grisea genome. A large-scale functional genomics project on M. grisea and its interaction with rice is also underway. It is an exciting time for genomics studies in M. grisea, and the resources generated in these studies will certainly enhance our understanding of fungal pathogenicity.
摘要稻瘟病菌（Magnaporthe grisea）引发了水稻最具破坏性的疾病之一。过去十年间，对这一重要病原体的遗传研究使其成为研究真菌-植物相互作用的优秀系统。最近，稻瘟病研究界的共同努力也使稻瘟病菌的基因组学研究取得了重大进展。BAC 连续片段已被组装并整合到高密度遗传图谱中，并且 12674 个 BAC 末端被测序作为基因组普查序列。通过散弹枪方法已经对超过十个 BAC 克隆进行了测序。对一个完全测序的 BAC 克隆的初步分析表明，稻瘟病菌可能具有 4.2kb/gene 的基因密度，并且包含大约 9000 个基因。此外，从代表真菌生长、分化和植物感染不同阶段的几个 cDNA 文库中已测序了 >10000 个 ESTs。目前，正在进行额外的 ESTs、端粒和 6X 覆盖率的测序项目。大规模的稻瘟病菌及其与水稻相互作用的功能基因组学项目也正在进行中。这是稻瘟病菌基因组学研究的激动人心时刻，这些研究产生的资源必将增强我们对真菌致病性的理解。
