稻瘟病菌代表性附着胞阶段cDNA文库。
Representative appressorium stage cDNA library of Magnaporthe grisea.
作者信息
Lu Jian-ping, Liu Tong-bao, Yu Xiao-yun, Lin Fu-cheng
机构信息
School of Life Sciences, Zhejiang University, Hangzhou 310029, China.
出版信息
J Zhejiang Univ Sci B. 2005 Feb;6(2):132-6. doi: 10.1631/jzus.2005.B0132.
Abstract
A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea, was constructed in a lambdaTriplEx2 vector by SMART cDNA library containing 2.37x10(6) independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the early stages of penetration of M. grisea.
摘要
利用SMART cDNA文库技术，在lambdaTriplEx2载体中构建了稻瘟病菌成熟附着胞的cDNA文库。该文库包含2.37×10⁶个独立克隆，其中约100%含有外源cDNA插入片段，平均大小为660 bp。在随机挑选的9个克隆中，有2个表达序列标签（ESTs）序列在GenBank中没有稻瘟病菌的同源EST序列。该附着胞cDNA文库适用于稻瘟病菌附着胞形成后期和侵入前期的基因表达分析和功能分析。
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本文引用的文献
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