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稻瘟病菌代表性附着胞阶段cDNA文库。

Representative appressorium stage cDNA library of Magnaporthe grisea.

作者信息

Lu Jian-ping, Liu Tong-bao, Yu Xiao-yun, Lin Fu-cheng

机构信息

School of Life Sciences, Zhejiang University, Hangzhou 310029, China.

出版信息

J Zhejiang Univ Sci B. 2005 Feb;6(2):132-6. doi: 10.1631/jzus.2005.B0132.

DOI:10.1631/jzus.2005.B0132
PMID:15633249
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1389628/
Abstract

A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea, was constructed in a lambdaTriplEx2 vector by SMART cDNA library containing 2.37x10(6) independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the early stages of penetration of M. grisea.

摘要

利用SMART cDNA文库技术，在lambdaTriplEx2载体中构建了稻瘟病菌成熟附着胞的cDNA文库。该文库包含2.37×10⁶个独立克隆，其中约100%含有外源cDNA插入片段，平均大小为660 bp。在随机挑选的9个克隆中，有2个表达序列标签（ESTs）序列在GenBank中没有稻瘟病菌的同源EST序列。该附着胞cDNA文库适用于稻瘟病菌附着胞形成后期和侵入前期的基因表达分析和功能分析。

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本文引用的文献

1
cDNA Subtractive Cloning of Genes Expressed during Early Stage of Appressorium Formation by Magnaporthe grisea.稻瘟病菌附着胞形成早期表达基因的cDNA消减克隆
Biosci Biotechnol Biochem. 1999;63(8):1407-13. doi: 10.1271/bbb.63.1407.
2
Genes expressed during early stages of rice infection with the rice blast fungus Magnaporthe grisea.在水稻感染稻瘟病菌（Magnaporthe grisea）的早期阶段表达的基因。
Mol Plant Pathol. 2001 Nov 1;2(6):347-54. doi: 10.1046/j.1464-6722.2001.00085.x.
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Large scale parallel analysis of gene expression during infection-related morphogenesis of Magnaporthe grisea.在稻瘟病菌感染相关形态发生过程中进行大规模平行的基因表达分析。
Mol Plant Pathol. 2003 Sep 1;4(5):337-46. doi: 10.1046/j.1364-3703.2003.00182.x.
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Time for a blast: genomics of Magnaporthe grisea. Blast 时间：灰霉菌的基因组学。
Mol Plant Pathol. 2002 May 1;3(3):173-6. doi: 10.1046/j.1364-3703.2002.00108.x.
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Serial Analysis of Gene Expression (SAGE) of Magnaporthe grisea: genes involved in appressorium formation.稻瘟病菌基因表达系列分析（SAGE）：与附着胞形成相关的基因
Mol Genet Genomics. 2003 Nov;270(2):181-9. doi: 10.1007/s00438-003-0911-6. Epub 2003 Aug 29.
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Cloning and characterization of genes specifically expressed during infection stages in the rice blast fungus.稻瘟病菌感染阶段特异性表达基因的克隆与特性分析
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7
MPG1 Encodes a Fungal Hydrophobin Involved in Surface Interactions during Infection-Related Development of Magnaporthe grisea.MPG1编码一种参与稻瘟病菌与感染相关发育过程中表面相互作用的真菌疏水蛋白。
Plant Cell. 1996 Jun;8(6):985-999. doi: 10.1105/tpc.8.6.985.
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Regulatory Genes Controlling MPG1 Expression and Pathogenicity in the Rice Blast Fungus Magnaporthe grisea.控制稻瘟病菌稻瘟菌MPG1表达和致病性的调控基因。
Plant Cell. 1996 May;8(5):771-781. doi: 10.1105/tpc.8.5.771.
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Two novel fungal virulence genes specifically expressed in appressoria of the rice blast fungus.两个在稻瘟病菌附着胞中特异性表达的新型真菌毒力基因。
Plant Cell. 2002 Sep;14(9):2107-19. doi: 10.1105/tpc.003426.
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A Magnaporthe grisea cyclophilin acts as a virulence determinant during plant infection.一种稻瘟病菌亲环蛋白在植物感染过程中作为毒力决定因素发挥作用。
Plant Cell. 2002 Apr;14(4):917-30. doi: 10.1105/tpc.010389.