Department of Cardiology, Second Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang 325000, China.
BMC Cardiovasc Disord. 2010 Jun 22;10:30. doi: 10.1186/1471-2261-10-30.
Development of experimental animal models has played an important role in understanding the mechanisms of cardiac memory. The purpose of this study was to evaluate a new canine model of cardiac memory using endocardial ventricular pacing via internal jugular vein.
Twelve Beagle dogs underwent placement of a permanent ventricular pacemaker mimicking the use of pacemakers in humans and induction of cardiac memory by endocardial ventricular pacing.
Cardiac memory was achieved in 11 of 12 attempts overall. Procedural mortality due to cardiac tamponade (n = 1) occurred in the first attempt. The T-wave memory persisted for 96 +/- 17 minutes and 31 +/- 6 days in the short-term and long-term cardiac memory groups, respectively. There were no significant differences in the heart rate, blood pressure and echocardiographic parameters in the animals between before and after ventricular pacing in the short-term and long-term cardiac memory groups. No significant pathologic changes with the light microscopy were found in the present study in all dogs.
The model does require surgery but is not as invasive as an open-chest model. This canine model can serve as a useful tool for studying mechanisms of cardiac memory.
实验动物模型的发展在理解心脏记忆机制方面发挥了重要作用。本研究旨在通过经颈内静脉心内膜心室起搏评估一种新的犬类心脏记忆模型。
12 只比格犬接受了永久性心室起搏器的植入，模拟了人类起搏器的使用，并通过心内膜心室起搏诱导了心脏记忆。
12 次尝试中有 11 次成功实现了心脏记忆。第一次尝试中因心脏压塞导致手术死亡率为 1 例。在短期和长期心脏记忆组中，T 波记忆分别持续了 96 ± 17 分钟和 31 ± 6 天。在短期和长期心脏记忆组中，起搏前后动物的心率、血压和超声心动图参数均无显著差异。在本研究中，所有犬的光镜下均未发现明显的病理变化。
该模型需要手术，但不如开胸模型具有侵袭性。这种犬类模型可以作为研究心脏记忆机制的有用工具。
