Department of Public Health and Caring Sciences/Clinical Nutrition and Metabolism, Uppsala University, Uppsala Science Park, 75185 Uppsala, Sweden.
Br J Nutr. 2010 Nov;104(9):1357-62. doi: 10.1017/S000711451000228X. Epub 2010 Jun 23.
Subjects with the metabolic syndrome (MetS) have enhanced oxidative stress and inflammation. Dietary fat quality has been proposed to be implicated in these conditions. We investigated the impact of four diets distinct in fat quantity and quality on 8-iso-PGF2α (a major F2-isoprostane and oxidative stress indicator), 15-keto-13,14-dihydro-PGF2α (15-keto-dihydro-PGF2α, a major PGF2α metabolite and marker of cyclooxygenase-mediated inflammation) and C-reactive protein (CRP). In a 12-week parallel multicentre dietary intervention study (LIPGENE), 417 volunteers with the MetS were randomly assigned to one of the four diets: two high-fat diets (38 % energy (%E)) rich in SFA or MUFA and two low-fat high-complex carbohydrate diets (28 %E) with (LFHCC n-3) or without (LFHCC) 1·24 g/d of very long chain n-3 fatty acid supplementation. Urinary levels of 8-iso-PGF2α and 15-keto-dihydro-PGF2α were determined by RIA and adjusted for urinary creatinine levels. Serum concentration of CRP was measured by ELISA. Neither concentrations of 8-iso-PGF2α and 15-keto-dihydro-PGF2α nor those of CRP differed between diet groups at baseline (P>0·07) or at the end of the study (P>0·44). Also, no differences in changes of the markers were observed between the diet groups (8-iso-PGF2α, P = 0·83; 15-keto-dihydro-PGF2α, P = 0·45; and CRP, P = 0·97). In conclusion, a 12-week dietary fat modification did not affect the investigated markers of oxidative stress and inflammation among subjects with the MetS in the LIPGENE study.
患有代谢综合征 (MetS) 的受试者存在氧化应激和炎症增强。饮食中的脂肪质量已被认为与这些情况有关。我们研究了四种不同脂肪量和质量的饮食对 8-异前列腺素 F2α（主要的 F2-异前列腺素和氧化应激标志物）、15-酮-13,14-二氢-PGF2α（15-酮-二氢-PGF2α，主要的 PGF2α 代谢物和环氧化酶介导的炎症标志物）和 C 反应蛋白 (CRP) 的影响。在一项为期 12 周的平行多中心饮食干预研究（LIPGENE）中，417 名患有代谢综合征的志愿者被随机分配到以下四种饮食中的一种：两种高脂肪饮食（38%能量 (%E)) 富含 SFA 或 MUFA，两种低脂肪高复合碳水化合物饮食（28%E)，其中含有（LFHCC n-3）或不含有（LFHCC）1·24 g/d 的非常长链 n-3 脂肪酸补充剂。通过 RIA 测定尿液中 8-异前列腺素 F2α 和 15-酮-二氢-PGF2α 的水平，并根据尿液肌酐水平进行调整。通过 ELISA 测量血清 CRP 浓度。在基线（P>0·07）或研究结束时（P>0·44），各组之间尿液中 8-异前列腺素 F2α 和 15-酮-二氢-PGF2α 的浓度以及 CRP 浓度均无差异（P>0·07）。此外，各组之间标记物的变化也没有差异（8-异前列腺素 F2α，P=0·83；15-酮-二氢-PGF2α，P=0·45；和 CRP，P=0·97）。总之，在 LIPGENE 研究中，12 周的饮食脂肪调整并未影响代谢综合征患者氧化应激和炎症的标志物。
