鲇形目鲇科胡鲶属鱼类（胡鲶，布洛赫）人工受精的优化研究

Optimization of artificial fertilization in the stinging catfish Heteropneustes fossilis (Bloch).

作者信息

Christopher J Godwin, Murugesan A G, Sukumaran N

机构信息

School of Biosciences and Technology, VIT University, Vellore-632 014, India.

出版信息

Zygote. 2011 Feb;19(1):63-6. doi: 10.1017/S0967199410000122. Epub 2010 Jun 23.

DOI:10.1017/S0967199410000122

PMID:20569508

Abstract

Determination of optimum sperm requirement in artificial breeding helps to improve the fertilization efficiency, sperm economy and minimize the sacrificing of males for milt. Quantification of sperm cells was done by spectrophotometer at 420 nm followed by haemocytometer counting. Best correlation (r2 = 0.97) was recorded. The minimum number of sperm required for optimal fertilization success in Heteropneustes fossilis was determined. An average fertilization success of 78-93% was recorded at 8 × 103 to 8 × 107 sperm per egg. The optimum contact duration of gametes was 5 minutes. The highest fertilization rate of 98.18% was recorded at 8 × 107 spermatozoa.egg-1.

摘要

确定人工繁殖中的最佳精子需求量有助于提高受精效率、精子利用率，并尽量减少为获取精液而宰杀雄鱼的情况。通过分光光度计在420纳米波长下进行精子细胞定量，随后用血细胞计数器计数。记录到最佳相关性（r2 = 0.97）。确定了印度胡鲶实现最佳受精成功率所需的最低精子数量。每枚卵有8×103至8×107个精子时，平均受精成功率为78 - 93%。配子的最佳接触持续时间为5分钟。精子数为8×107个/卵时，记录到最高受精率为98.18%。