Yron I, Langer A, Weinstein T, Sahar E, Lidor Y, Pardo Y, Katz I, Shohat L, Kalechman Y, Ovadia J
Department of Microbiology, Moise and Frida Eskenasy Institute for Cancer Research, Tel Aviv, Israel.
Nat Immun Cell Growth Regul. 1991;10(1):32-44.
The effect of sex hormones on concanavalin A (Con A)-activated human T cells was studied. We show that neither 17 beta-estradiol (E2) nor progesterone, in concentrations of up to 10(-6) M, alters the proliferative response of peripheral-blood mononuclear cells (PBMC) of healthy postmenopausal women. Furthermore, the hormones had no effect on the composition of T cell populations and on the expression of activation markers. We extended our study to a unique T cell population that is characterized by the ability to form rosettes with human erythrocytes, following Con A activation (designated autorosette-forming cells; ARFC) and known to manifest suppressive activity. Indeed, the in vitro addition of E2 (neither progesterone nor testosterone) to Con A-stimulated PBMC brought an about 2- to 4-fold increase in the frequency of ARFC. Tamoxifen, an antiestrogen drug, reduced the frequency of estrogen-stimulated ARFC to the original low level. Furthermore, the inhibitory effect of growth medium from ARFC cultures originally stimulated with Con A + E2 was found to be higher than that of ARFC cultures originally stimulated with Con A alone.
研究了性激素对伴刀豆球蛋白A（Con A）激活的人T细胞的影响。我们发现，浓度高达10⁻⁶ M的17β-雌二醇（E2）和孕酮均不会改变健康绝经后妇女外周血单个核细胞（PBMC）的增殖反应。此外，这些激素对T细胞群体的组成以及激活标志物的表达均无影响。我们将研究扩展至一个独特的T细胞群体，该群体在Con A激活后能够与人红细胞形成玫瑰花结（称为自身玫瑰花结形成细胞；ARFC），并且已知具有抑制活性。实际上，在Con A刺激的PBMC中体外添加E2（而非孕酮或睾酮）可使ARFC的频率增加约2至4倍。抗雌激素药物他莫昔芬可将雌激素刺激的ARFC频率降低至原始低水平。此外，发现最初用Con A + E2刺激的ARFC培养物的生长培养基的抑制作用高于最初仅用Con A刺激的ARFC培养物。
