University Department of Growth and Reproduction, Copenhagen University Hospital, GR 5064, Blegdamsvej 9, DK-2100 Copenhagen, Denmark.
Hum Reprod. 2010 Aug;25(8):2031-8. doi: 10.1093/humrep/deq147. Epub 2010 Jun 22.
Cryopreservation of semen should be offered to adults before gonadotoxic treatment. However, the experience with semen collection in adolescents is still limited. The objective of this study was to evaluate potential correlates of successful semen sampling in adolescents.
A total of 86 boys (aged 12.2-17.9 years), referred for cryopreservation of semen prior to gonadotoxic treatment were included. Age, testicular volume, diagnosis and reproductive hormones were evaluated as correlates of successful semen collection.
Median sperm concentration was 9.6 (range 0-284) million/ml. Of the 86 included boys, 76 (88.4%) had spermatozoa in their ejaculate. Of the 76 patients for whom a semen sample was obtained, 71 (93.4%) had motile spermatozoa eligible for cryopreservation. Of the 86 boys, 74 produced a semen sample by masturbation, whereas semen samples were obtained from 12 patients by penile vibration or electroejaculation. The youngest patient with an ejaculate containing motile spermatozoa was 12.2 years old, and the smallest testicular volumes in boys associated with motile spermatozoa in the ejaculate were 6-7 ml. Testicular volume correlated with sperm concentration (R = 0.283, P = 0.046), and the percentage of motile spermatozoa (R = 0.410, P = 0.003). Chronological age, but not reproductive hormones, also correlated with sperm concentration (R = 0.25, P = 0.049).
Semen was successfully collected and cryopreserved in 71 out of 86 boys and adolescents. Testicular volume, but not age or reproductive hormone levels, was indicative of successful semen collection. Regardless of their age, adolescent boys with testicular volumes of more than 5 ml should be offered semen banking prior to gonadotoxic treatment or other procedures that could potentially damage future fertility.
在性腺毒性治疗前，应向成年人提供精液冷冻保存。然而，青少年的精液采集经验仍然有限。本研究的目的是评估青少年成功精液采样的潜在相关因素。
共纳入 86 名（年龄 12.2-17.9 岁）因性腺毒性治疗前冷冻保存精液而就诊的男孩。评估年龄、睾丸体积、诊断和生殖激素与成功精液采集的相关性。
中位精子浓度为 9.6（范围 0-284）百万/ml。在 86 名纳入的男孩中，76 名（88.4%）的精液中有精子。在获得精液样本的 76 名患者中，71 名（93.4%）有可用于冷冻保存的活动精子。在 86 名男孩中，74 名通过手淫产生了精液样本，而 12 名患者通过阴茎振动或电刺激获得了精液样本。有活动精子的精液样本的最小年龄为 12.2 岁，睾丸体积最小的男孩的睾丸体积为 6-7 ml。睾丸体积与精子浓度相关（R = 0.283，P = 0.046），与精子活力相关（R = 0.410，P = 0.003）。实际年龄与精子浓度相关（R = 0.25，P = 0.049），但与精子活力无关。
在 86 名男孩和青少年中，成功采集并冷冻保存了 71 份精液。睾丸体积，但不是年龄或生殖激素水平，是成功采集精液的指标。无论年龄大小，睾丸体积大于 5ml 的青少年男孩在接受性腺毒性治疗或其他可能损害未来生育能力的治疗前，应提供精液库存储。
