吸入香烟烟雾可调节骨愈合部位的基因表达：一项大鼠研究。
Cigarette smoke inhalation modulates gene expression in sites of bone healing: a study in rats.
作者信息
Giorgetti Ana Paula Oliveira, César Neto João Batista, Ruiz Karina Gonzales Silvério, Casati Márcio Zaffalon, Sallum Enílson Antonio, Nociti Francisco Humberto
机构信息
Periodontics Division, Department of Prosthodontics and Periodontics, School of Dentistry at Piracicaba, State University of Campinas, Piracicaba, São Paulo, Brazil.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Oct;110(4):447-52. doi: 10.1016/j.tripleo.2010.02.029. Epub 2010 Jun 23.
OBJECTIVE
The aim of this study was to assess the effect of cigarette smoke inhalation (CSI) on gene expression in alveolar bone healing sites.
STUDY DESIGN
Wistar rats were randomly assigned to the groups: control [animals not exposed to CSI (n = 20)] and test [animals exposed to CSI, starting 3 days before teeth extraction and maintained until killing them (n = 20)]. First mandibular molars were bilaterally extracted, and the expression of alkaline phosphatase, bone morphogenetic protein (BMP) 2 and 7, receptor activator of nuclear factor κB ligand, osteoprotegerin, and d2 isoform of vacuolar adenosine triphosphatase V(0) domain were assessed by quantitative polymerase chain reaction in the newly formed tissue in the sockets.
RESULTS
Overall, data analysis demonstrated that CSI significantly affected the expression pattern of all of the studied genes except BMP-7.
CONCLUSION
The expression of key genes for bone healing may be affected by CSI in tooth extraction sites.
目的
本研究旨在评估吸入香烟烟雾（CSI）对牙槽骨愈合部位基因表达的影响。
研究设计
将Wistar大鼠随机分为两组：对照组[未暴露于CSI的动物（n = 20）]和试验组[从拔牙前3天开始暴露于CSI并持续至处死的动物（n = 20）]。双侧拔除第一下颌磨牙，通过定量聚合酶链反应评估牙槽窝新形成组织中碱性磷酸酶、骨形态发生蛋白（BMP）2和7、核因子κB受体活化因子配体、骨保护素以及液泡型腺苷三磷酸酶V(0)结构域d2亚型的表达。
结果
总体而言，数据分析表明，除BMP - 7外，CSI显著影响所有研究基因的表达模式。
结论
拔牙部位的骨愈合关键基因表达可能受CSI影响。
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