Service de Pharmacologie et d'Immuno-analyse, DSV/iBiTec-S, CEA/Saclay, 91191 Gif-sur-Yvette cedex, France.
Analyst. 2010 Sep;135(9):2203-19. doi: 10.1039/c0an00021c. Epub 2010 Jun 24.
The metabolome is characterized by a large number of molecules exhibiting a high diversity of chemical structures and abundances, requiring complementary analytical platforms for extensive coverage. Of these analytical platforms, atmospheric pressure ionization Fourier transform mass spectrometry (FT/MS) instruments are popular because they provide accurate mass measurements with ppm and even sub-ppm errors, and also high and ultra-high resolving power. In this article, we evaluate the improvements provided for metabolomics by different types of FT/MS instruments, together with the ability of these platforms to cover the various analytical requirements: global metabolite profiling, absolute quantification and also structural characterization, of metabolomics. The specificities of FT/MS in terms of data pre-processing and the input of accurate mass measurements for biological interpretation and for highlighting metabolic networks are also addressed.
代谢组学的特点是存在大量分子，这些分子具有高度多样化的化学结构和丰度，需要互补的分析平台来进行广泛的覆盖。在这些分析平台中，大气压电离傅里叶变换质谱（FT/MS）仪器很受欢迎，因为它们提供了具有 ppm 甚至亚 ppm 误差的精确质量测量，以及高分辨率和超高分辨率。在本文中，我们评估了不同类型的 FT/MS 仪器为代谢组学提供的改进，以及这些平台覆盖各种分析要求的能力：全局代谢物分析、绝对定量以及代谢组学的结构特征。还讨论了 FT/MS 在数据预处理和输入精确质量测量方面的特点，这些特点对于生物学解释和突出代谢网络具有重要意义。
