Baidoo Edward E K, Benke Peter I, Keasling Jay D
Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Methods Mol Biol. 2012;881:215-78. doi: 10.1007/978-1-61779-827-6_9.
Through the characterization of metabolic pathways, metabolomics is able to illuminate the activities of a cell at the functional level. However, the metabolome, which is comprised of hundreds of chemically diverse metabolites, is rather difficult to monitor. Mass spectrometry (MS)-based profiling methods are increasingly being utilized for this purpose. To this end, MS is often coupled to the separation techniques gas chromatography (GC), liquid chromatography (LC), and capillary electrophoresis (CE). The mass-based selectivity that the MS provides, together with the chromatographic or electrophoretic separation of analytes, creates hyphenated techniques that are ideally suited to the measurement of large numbers of metabolites from microbial extracts. In this chapter, we describe GC-MS, LC-MS, and CE-MS methods that are applicable to microbial metabolomics experiments.
通过对代谢途径的表征，代谢组学能够在功能水平上阐明细胞的活动。然而，由数百种化学性质各异的代谢物组成的代谢组相当难以监测。基于质谱（MS）的分析方法正越来越多地用于此目的。为此，质谱通常与气相色谱（GC）、液相色谱（LC）和毛细管电泳（CE）等分离技术联用。质谱提供的基于质量的选择性，与分析物的色谱或电泳分离相结合，产生了非常适合于测量微生物提取物中大量代谢物的联用技术。在本章中，我们描述了适用于微生物代谢组学实验的气相色谱-质谱、液相色谱-质谱和毛细管电泳-质谱方法。
