Advanced Urogenital Disease, Chung-Ang University College of Medicine, Seoul, Korea.
BJU Int. 2011 Jan;107(1):144-9. doi: 10.1111/j.1464-410X.2010.09469.x.
To investigate the expression and regulation of human β-defensin-2 (HBD-2) in the prostate.
Normal human prostate epithelial cell line (RWPE-1), human prostate cancer cell lines (DU-145, PC-3), and paraffin-embedded prostate tissue from patients with benign prostatic hyperplasia (BPH) were analysed by RT-PCR and immunohistochemical staining. HBD-2 expression was also analysed by RT-PCR and ELISA in RWPE-1 cells treated with lipopolysaccharide (LPS). Nuclear factor-κB (NF-κB) activation was assessed by IκBα immunoblotting and electrophoretic mobility shift assay (EMSA).
BPH tissue and all of the tested prostate cell lines other than PC-3 constitutively express HBD-2 mRNA. HBD-2 protein was strongly detected in prostate gland tissue surrounded by inflammatory cells including macrophages. Exposure to LPS induced HBD-2 upregulation and NF-κB activation, as assessed by IκBα phosphorylation and degradation in RWPE-1 cells. Bay11-7082, an NF-κB inhibitor prevented LPS-induced HBD-2 production in RWPE-1 cells.
Prostate epithelial cells may constitutively express HBD-2, and its expression was upregulated by LPS. Our data indicate that HBD-2 may be an important immunomodulatory factor in prostate function. Expression of HBD-2 in normal prostates and the potential role of HBD-2 in prostatitis and BPH should be addressed in the future.
研究人β防御素-2（HBD-2）在前列腺中的表达和调控。
应用 RT-PCR 和免疫组织化学染色分析正常前列腺上皮细胞系（RWPE-1）、人前列腺癌细胞系（DU-145、PC-3）和前列腺增生症（BPH）患者石蜡包埋组织中 HBD-2 的表达。还通过 LPS 处理的 RWPE-1 细胞分析 HBD-2 的表达。通过 IκBα 免疫印迹和电泳迁移率变动分析（EMSA）评估核因子-κB（NF-κB）的激活。
BPH 组织和除 PC-3 之外的所有测试前列腺细胞系均持续表达 HBD-2 mRNA。HBD-2 蛋白在前列腺组织中强烈检测到，这些组织被包括巨噬细胞在内的炎症细胞所包围。用 LPS 处理 RWPE-1 细胞，通过 IκBα 磷酸化和降解，可诱导 HBD-2 上调和 NF-κB 激活。NF-κB 抑制剂 Bay11-7082 可防止 LPS 诱导 RWPE-1 细胞产生 HBD-2。
前列腺上皮细胞可能持续表达 HBD-2，其表达可被 LPS 上调。我们的数据表明，HBD-2 可能是前列腺功能的一个重要免疫调节因子。在未来，应研究 HBD-2 在正常前列腺中的表达及其在前列腺炎和 BPH 中的潜在作用。
