Justus-Liebig Universität Giessen, Institut für Angewandte Mikrobiologie, 35392 Giessen, Germany.
Syst Appl Microbiol. 2010 Aug;33(5):260-8. doi: 10.1016/j.syapm.2010.04.006. Epub 2010 Jun 26.
Mould-colonized water damaged building materials are frequently co-colonized by actinomycetes. Here, we report the results of the analyses of Actinobacteria on different wall materials from water damaged buildings obtained by both cultivation-dependent and cultivation-independent methods. Actinobacteria were detected in all but one of the investigated materials by both methods. The detected concentrations of Actinobacteria ranged between 1.8 x 10(4) and 7.6 x 10(7) CFUg(-1) of investigated material. A total of 265 isolates from 17 materials could be assigned to 31 different genera of the class Actinobacteria on the basis of 16S rRNA gene sequence analyses. On the basis of the cultivation-independent approach, 16S rRNA gene inserts of 800 clones (50%) were assigned to 47 different genera. Representatives of the genera Streptomyces, Amycolatopsis, Nocardiopsis, Saccharopolyspora, Promicromonospora, and Pseudonocardia were found most frequently. The results derived from both methods indicated a high abundance and variety of Actinobacteria in water damaged buildings. Four bioaerosol samples were investigated by the cultivation-based approach in order to compare the communities of Actinobacteria in building material and associated air samples. A comparison of the detected genera of bioaerosol samples with those directly obtained from material samples resulted in a congruent finding of 9 of the overall 35 detected genera (25%), whereas four genera were only detected in bioaerosol samples.
受霉菌污染的水损坏建筑材料经常被放线菌共同污染。在这里，我们报告了通过培养依赖和非培养方法从受水损坏建筑物获得的不同墙壁材料上放线菌分析的结果。通过这两种方法，在所研究的材料中，除一种外，其余的都检测到了放线菌。所检测到的放线菌浓度在 1.8 x 10(4)到 7.6 x 10(7) CFUg(-1)的调查材料之间。在基于 16S rRNA 基因序列分析的基础上，从 17 种材料中的 265 个分离物可被分配到类放线菌的 31 个不同属。基于非培养方法，800 个克隆（50%）的 16S rRNA 基因插入物被分配到 47 个不同的属。链霉菌属、拟无枝酸菌属、诺卡氏菌属、糖多孢菌属、原小单孢菌属和假诺卡氏菌属最为常见。两种方法的结果均表明，水损坏建筑物中的放线菌数量丰富，种类多样。通过培养方法对四个生物气溶胶样本进行了调查，以便比较建筑材料和相关空气样本中放线菌群落。将直接从材料样本中检测到的生物气溶胶样本的检测属与生物气溶胶样本的检测属进行比较，发现 35 个总体检测属中的 9 个（25%）具有一致性，而四个属仅在生物气溶胶样本中检测到。
