Department of Pathophysiology, Jessenius Faculty of Medicine in Martin, Commenius University in Bratislava, Sklabinska Str. 26, 036 01 Martin, Slovak Republic.
Respir Physiol Neurobiol. 2010 Aug 31;173(1):11-5. doi: 10.1016/j.resp.2010.05.015. Epub 2010 May 16.
Central neuronal interaction seems to play a role in pathogenesis of upper airway cough syndrome. In the guinea pig model we used the method c-fos expression to identify neurons involved in processing of nociceptive nasal stimuli and their contribution to enhancement of cough. 21 spontaneously breathing, urethane anaesthetized animals were used. The controls received intranasal saline, stimulation group received capsaicin (15 microl, 50 microM), and not-treated group was free of nasal challenge. After 2 h animals were deeply anaesthetized, exsanguinated and transcardially perfused with saline and paraformaldehyde. The brainstems were removed, post-fixed, and slices were processed immunohistochemically for c-fos. In capsaicin group the FLI was detected in the nTs 0.5 mm caudal, 1.5 mm lateral to the obex, the area postrema, LRN and VRG. Intensive FLI was identified in trigeminal nuclear complex. Mean number of FOS positive neurons per section was significantly higher in capsaicin group than that in no-treatment controls or saline controls at the level of obex (p<0.01). Neurons of nTs and VRG clearly activated after nasal provocation may participate in enhancement of cough.
中枢神经元相互作用似乎在上气道咳嗽综合征的发病机制中起作用。在我们使用 c-fos 表达方法的豚鼠模型中，鉴定了参与处理鼻内伤害性刺激的神经元及其对咳嗽增强的贡献。共使用 21 只自主呼吸、氨基甲酸乙酯麻醉的动物。对照组接受鼻内生理盐水，刺激组接受辣椒素（15 微升，50 microM），未处理组无鼻内刺激。2 小时后，动物被深度麻醉，放血，并用生理盐水和多聚甲醛进行心脏灌注。取出脑干，后固定，并用免疫组织化学方法对 c-fos 进行切片处理。在辣椒素组中，在延髓腹外侧（obex）后 0.5 毫米、外侧 1.5 毫米处的 nTs、极后区、LRN 和 VRG 中检测到 FLI。三叉神经核复合体中鉴定出强烈的 FLI。在 obex 水平，辣椒素组每节的 FOS 阳性神经元数明显高于未处理对照组或生理盐水对照组（p<0.01）。鼻内刺激后 nTs 和 VRG 的神经元明显被激活，可能参与咳嗽的增强。
