Jeeno Peerapong, Tongban Sukit, Yana Pichamon, Wongta Anurak, Sutan Kunrunya, Yadoung Sumed, Hongsibsong Surat
School of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Chemistry, Faculty of Science and Technology, Chiang Mai Rajabhat University, Chiang Mai 50300, Thailand.
Plants (Basel). 2022 Aug 11;11(16):2089. doi: 10.3390/plants11162089.
The Smilacaceae family has been used as a food source and herbal medicine for a long time. This study aims to identify the phytochemicals extracted from and by using LC-QTOF/MS analysis and determine their bioactive potential. Compounds were identified from and extracts by LC-QTOF-MS and it was found that longistylin A and CAY10435 have higher degrees of matching compounds (99.66% and 99.87%). showed antioxidant capacity, i.e., DPPH and ABTS at percentage inhibitions of 71.94 ± 1.46% and 59.84 ± 4.80%, respectively, and FRAP at 730.69 ± 33.62 mg AAE/100 g sample. The total phenolic compound contents of the ethanol, methanol, and water extracts were 0.017 ± 0.001, 0.015 ± 0.001, and 0.016 ± 0.001 mg GAE/g, respectively, while the total flavonoid contents were 0.043 ± 0.002, 0.033 ± 0.002, and 0.006 ± 0.003 mg QE/g, respectively. The anti-inflammatory capacity showed 97.26% protection and 2.74% hemolysis. The antimicrobial activity can inhibit Gram-positive bacteria with a minimum inhibitory concentration (MIC) of 62.5 mg/mL and a minimum bactericidal concentration (MBC) of 500 mg/mL. showed antioxidant capacity, i.e., DPPH and ABTS at percentage inhibitions of 72.24 ± 0.64% and 39.87 ± 2.37%, respectively, and FRAP at 208.33 ± 50.80 mg AAE/100 g sample. The total phenolic compound contents of the ethanol, methanol, and water extracts were 0.006 ± 0.000, 0.007 ± 0.002, and 0.002 ± 0.001 mg GAE/g, respectively, while the total flavonoid contents of the ethanol and methanol extracts were 0.012 ± 0.001 and 0.008 ± 0.000 mg QE/g, respectively. The anti-inflammatory capacity showed 96.64% protection and 3.36% hemolysis. The antimicrobial activity of the extracts can inhibit Gram-positive bacteria with a MIC of 31.25 mg/mL and MBC of 125 mg/mL for the ethanol extract and a MIC of 125 mg/mL and MBC of 62.5 mg/mL for the methanol extract. In conclusion, and were found to contain several phytochemicals that can be used for further study. Both Smilax species can also be used as sources of antioxidants and herbal medicines for killing Gram-positive bacteria.
菝葜科长期以来一直被用作食物来源和草药。本研究旨在通过液相色谱-四极杆飞行时间质谱（LC-QTOF/MS）分析鉴定从[具体植物1]和[具体植物2]中提取的植物化学物质，并确定它们的生物活性潜力。通过LC-QTOF-MS从[具体植物1]和[具体植物2]提取物中鉴定出化合物，发现长柱菝葜素A和CAY10435具有较高的匹配度（分别为99.66%和99.87%）。[具体植物1]表现出抗氧化能力，即对二苯基苦味酰基自由基（DPPH）和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）的抑制率分别为71.94±1.46%和59.84±4.80%，铁离子还原抗氧化能力（FRAP）为730.69±33.62毫克抗坏血酸当量/100克样品。乙醇、甲醇和水提取物中总酚类化合物含量分别为0.017±0.001、0.015±0.001和0.016±0.001毫克没食子酸当量/克，而总黄酮含量分别为0.043±0.002、0.033±0.002和0.006±0.003毫克芦丁当量/克。抗炎能力表现为97.26%的保护率和2.74%的溶血率。抗菌活性可抑制革兰氏阳性菌，最低抑菌浓度（MIC）为62.5毫克/毫升，最低杀菌浓度（MBC）为500毫克/毫升。[具体植物2]表现出抗氧化能力，即对DPPH和ABTS的抑制率分别为72.24±0.64%和39.87±2.37%，FRAP为208.33±50.80毫克抗坏血酸当量/100克样品。乙醇、甲醇和水提取物中总酚类化合物含量分别为0.006±0.000、0.007±0.002和0.002±0.001毫克没食子酸当量/克，而乙醇和甲醇提取物中总黄酮含量分别为0.012±0.001和0.008±0.000毫克芦丁当量/克。抗炎能力表现为96.64%的保护率和3.36%的溶血率。提取物的抗菌活性对乙醇提取物可抑制革兰氏阳性菌，MIC为31.25毫克/毫升，MBC为125毫克/毫升；对甲醇提取物，MIC为125毫克/毫升，MBC为62.5毫克/毫升。总之，发现[具体植物1]和[具体植物2]含有几种可用于进一步研究的植物化学物质。两种菝葜属植物还可作为抗氧化剂来源和用于杀灭革兰氏阳性菌的草药。
