Laboratório de Helmintologia e Malacologia Médica, Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, Minas Gerais, Brazil.
Mol Phylogenet Evol. 2010 Oct;57(1):144-51. doi: 10.1016/j.ympev.2010.05.019. Epub 2010 May 23.
In Brazil, there are three intermediate snail vectors and two potential hosts of Schistosoma mansoni. Previous studies showed three variant molecular profiles to B. amazonica and evidenced intraspecific variations using sequence data. In this context, the aim of this study was to verify whether such differences would correspond to either B. amazonica or B. cousini. The snails were morphologically identified; PCR-RFLP and sequencing were carried out. Besides, B. cousini were submitted to susceptibility experiments to S. mansoni. Noteworthy, morphological data of Brazilian specimens predominantly showed the morphology described for B. amazonica. Nevertheless, PCR-RFLP results exhibited three variant molecular profiles for the specimens previously identified as B. amazonica and the phylogenetic analyses showed two groups one to B. amazonica and another to B. cousini. Furthermore, B. cousini showed to be susceptible to S. mansoni. These results confirm the occurrence of B. cousini in Brazil and points to the risk of introduction of schistosomiasis mansoni into new areas.
在巴西，有三种中间性蜗牛媒介和两种曼氏血吸虫的潜在宿主。先前的研究显示，B. amazonica 存在三种变异分子谱，并利用序列数据证明了种内变异。在这种情况下，本研究旨在验证这些差异是否对应于 B. amazonica 或 B. cousini。蜗牛通过形态学进行了鉴定；进行了 PCR-RFLP 和测序。此外，还对 B. cousini 进行了曼氏血吸虫易感性实验。值得注意的是，巴西标本的形态学数据主要显示了先前描述的 B. amazonica 的形态。然而，PCR-RFLP 结果显示，先前鉴定为 B. amazonica 的标本存在三种变异分子谱，系统发育分析显示，两组分别对应于 B. amazonica 和 B. cousini。此外，B. cousini 对曼氏血吸虫表现出易感性。这些结果证实了巴西存在 B. cousini，并指出了曼氏血吸虫病传入新地区的风险。
