利用光学相干断层扫描对哺乳动物胚胎早期发育过程进行活体成像。
Live Imaging of Early Developmental Processes in Mammalian Embryos with Optical Coherence Tomography.
作者信息
Larin Kirill V, Larina Irina V, Liebling Michael, Dickinson Mary E
机构信息
Department of Biomedical Engineering, University of Houston, Houston, TX, USA.
出版信息
J Innov Opt Health Sci. 2009 Jan 1;2(3):253-259. doi: 10.1142/S1793545809000619.
Abstract
Early embryonic imaging of cardiovascular development in mammalian models requires a method that can penetrate through and distinguish the many tissue layers with high spatial and temporal resolution. In this paper we evaluate the capability of Optical Coherence Tomography (OCT) technique for structural 3D embryonic imaging in mouse embryos at different stages of the developmental process ranging from 7.5 dpc up to 10.5 dpc. Obtained results suggest that the collected data is suitable for quantitative and qualitative measurements to assess cardiovascular function in mouse models, which is likely to expand our knowledge of the complexity of the embryonic heart, and its development into an adult heart.
摘要
在哺乳动物模型中对心血管发育进行早期胚胎成像，需要一种能够穿透并以高空间和时间分辨率区分众多组织层的方法。在本文中，我们评估了光学相干断层扫描（OCT）技术对发育过程中不同阶段（从胚胎发育7.5天到10.5天）的小鼠胚胎进行3D结构成像的能力。获得的结果表明，所收集的数据适用于定量和定性测量，以评估小鼠模型中的心血管功能，这可能会扩展我们对胚胎心脏复杂性及其向成体心脏发育的认识。
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