Bradu Adrian, Ma Lisha, Bloor James W, Podoleanu Adrian
Applied Optics Group, School of Physical Sciences, University of Kent, CT2 7NH Canterbury, UK.
J Biophotonics. 2009 Jul;2(6-7):380-8. doi: 10.1002/jbio.200910021.
This article demonstrates a combined instrument of two imaging modalities to acquire information on cardiac function in larval Drosophila melanogaster: optical coherence tomography (OCT) and laser scanning fluorescence microscopy (LSFM). For this purpose, a dedicated imaging instrument able to sequentially provide cross-sectional OCT and C-scan LSFM images has been developed. With this dual-imaging system, the heart can be easily located and visualized within the specimen and the change of the heart shape in a cardiac cycle can be monitored.
本文展示了一种结合两种成像方式的仪器，用于获取黑腹果蝇幼虫心脏功能的信息：光学相干断层扫描（OCT）和激光扫描荧光显微镜（LSFM）。为此，已开发出一种能够依次提供横截面OCT图像和C扫描LSFM图像的专用成像仪器。利用这种双成像系统，可以轻松在标本中定位并可视化心脏，并监测心动周期中心脏形状的变化。
