Potu Bhagath Kumar, Rao Muddanna S, Nampurath Gopalan Kutty, Chamallamudi Mallikarjuna Rao, Nayak Soubhagya Ranjan, Thomas Huban
Department of Anatomy, Centre for Basic Sciences, Kasturba Medical College, Manipal University, Manipal, Karnataka, India.
Chang Gung Med J. 2010 May-Jun;33(3):252-7.
The plant Cissus quadrangularis (CQ) is used as an osteoprotective agent in Ayurveda, the Indian system of alternative medicine. The present study was done to validate the anti-osteoporotic role of the petroleum ether extract of CQ on ovariectomy-induced osteoporosis in rats.
Female wistar rats were divided into five groups of six rats each; (1) The Normal control (NC) group (n=6) received no surgery and no treatment, (2). The Sham control (SHAM) group (n=6) received sham surgery but no treatment, (3) the Ovariectomized (OVX) group (n=6) received an ovariectomy and normal saline treatment for 90 days, (4) the Ovariectomized+raloxifene (OVX+RAL) group (n=6) received an ovariectomy and treatment with raloxifene, a known anti-osteoporotic agent for 90 days commencing from 22nd post ovariectomy day and (5) the Ovariectomy+Cissus quadrangularis (OVX+CQ) group (n=6) received an ovariectomy, and treatmentwith the petroleum ether extract of CQ 500 mg/kg body weight daily for 90 days commencing from 22nd post ovariectomy day. At the end of the treatment period, rats in all groups were sacrificed and the right femur was used for biomechanical analysis, and the left femur for histomorphometrical analysis.
CQ significantly increased the force required to break the femur (p<0.001) and significantly increased the thickness of both cortical (p<0.001) and trabecular bone (p<0.001). This action of CQ was comparable to the action of raloxifene.
The petroleum ether extract of CQ stem seems to possess anti-osteoporotic activity in rats.
在印度传统替代医学阿育吠陀中，四角旋花（CQ）被用作一种骨质保护剂。本研究旨在验证CQ石油醚提取物对大鼠去卵巢诱导的骨质疏松症的抗骨质疏松作用。
将雌性Wistar大鼠分为五组，每组六只；（1）正常对照组（NC）（n = 6）不进行手术且不接受治疗，（2）假手术对照组（SHAM）（n = 6）接受假手术但不接受治疗，（3）去卵巢组（OVX）（n = 6）接受去卵巢手术并给予生理盐水治疗90天，（4）去卵巢+雷洛昔芬组（OVX+RAL）（n = 6）接受去卵巢手术，并从去卵巢术后第22天开始用已知的抗骨质疏松药物雷洛昔芬治疗90天，（5）去卵巢+四角旋花组（OVX+CQ）（n = 6）接受去卵巢手术，并从去卵巢术后第22天开始每天给予500mg/kg体重的CQ石油醚提取物治疗90天。治疗期结束时，处死所有组的大鼠，右侧股骨用于生物力学分析，左侧股骨用于组织形态计量学分析。
CQ显著增加了股骨骨折所需的力（p<0.001），并显著增加了皮质骨（p<0.001）和小梁骨（p<0.001）的厚度。CQ的这种作用与雷洛昔芬的作用相当。
CQ茎的石油醚提取物似乎在大鼠中具有抗骨质疏松活性。
