Tasadduq Raazia, Gordon Jonathan, Al-Ghanim Khalid A, Lian Jane B, Van Wijnen Andre J, Stein Janet L, Stein Gary S, Shakoori Abdul Rauf
School of Biological Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore, Pakistan.
Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vemont.
J Cell Physiol. 2017 Mar;232(3):540-547. doi: 10.1002/jcp.25449. Epub 2016 Jun 15.
Traditional medicinal literature and previous studies have reported the possible role of Cissus quadrangularis (CQ) as an anti-osteoporotic agent. This study examines the effectiveness of CQ in promoting osteoblast differentiation of the murine pre-osteoblast cell line, MC3T3-E1. Ethanolic extract of CQ (CQ-E) was found to affect growth kinetics of MC3T3-E1 cells in a dosage-dependent manner. High concentrations of CQ-E (more than 10 μg/ml) have particularly adverse effects, while lower concentrations of 0.1 and 1 µg/ml were non-toxic and did not affect cell viability. Notably, cell proliferation was significantly increased at the lower concentrations of CQ-E. CQ-E treatment also augmented osteoblast differentiation, as reflected by a substantial increase in expression of the early osteoblast marker ALP activity, and at later stage, by mineralization of extracellular matrix compared to the control group. These findings suggest dose-dependent effect of CQ-E with lower concentrations exhibiting anabolic and osteogenic properties. J. Cell. Physiol. 232: 540-547, 2017. © 2016 Wiley Periodicals, Inc.
传统医学文献和先前的研究报道了四角金虎尾（CQ）作为一种抗骨质疏松药物的潜在作用。本研究考察了CQ促进小鼠前成骨细胞系MC3T3-E1成骨细胞分化的有效性。发现CQ的乙醇提取物（CQ-E）以剂量依赖的方式影响MC3T3-E1细胞的生长动力学。高浓度的CQ-E（超过10μg/ml）具有特别的不利影响，而较低浓度的0.1和1μg/ml无毒且不影响细胞活力。值得注意的是，较低浓度的CQ-E可显著增加细胞增殖。与对照组相比，CQ-E处理还增强了成骨细胞分化，这表现为早期成骨细胞标志物碱性磷酸酶（ALP）活性表达大幅增加，在后期则表现为细胞外基质矿化。这些发现表明CQ-E具有剂量依赖性效应，较低浓度表现出合成代谢和成骨特性。《细胞生理学杂志》2017年第232卷：540 - 547页。©2016威利期刊公司。
