Srikanta Praveen, Nagarajappa Shivaprasad H, Viswanatha Gollapalle L, Handral Mukund, Subbanna Rajesh, Srinath Rangappa, Hiremath Ganesh
Department of Pharmacology, People's Education Society College of Pharmacy, Bangalore, India.
Zhong Xi Yi Jie He Xue Bao. 2011 Oct;9(10):1125-32. doi: 10.3736/jcim20111014.
The present study was aimed to evaluate the anti-osteoporotic activity of methanolic extract of NR/CAL/06 in ovariectomized (OVX) rats.
Bilateral ovariectomy was performed in female Sprague-Dawley rats under aseptic conditions and the rats were divided into five groups (n=10). Two different doses of methanolic extract of NR/CAL/06 (200 and 400 mg/kg, per oral (p.o.)) were evaluated for anti-osteoporotic activity and raloxifene (5.4 mg/kg, p.o.) was used as a reference standard. Treatment was given for 90 d. Anti-osteoporotic potential of NR/CAL/06 was evaluated based on various parameters, namely, body weight, organ weight, bone weight, bone mineral content, bone strength, calcium and phosphorus in serum and urine, and serum alkaline phosphatase (ALP). External diameter, length and thickness of the femur bone were measured by using scanning electron microscopy (SEM).
The bilateral ovariectomy in rats resulted in decreased bone strength, bone mineral content and bone weight. The SEM images showed porous, perforated and disintegrated femur bone architecture and decreases in bone length and thickness in OVX rats. These changes were associated with elevated serum levels of calcium, phosphorus and ALP. Increases in body weight and adipose weight and a decrease in uterine weight were also observed and the changes were highly significant when compared with the sham-control group. Treatment with methanolic extract of NR/CAL/06 (200 and 400 mg/kg, p.o.) for 90 d dose-dependently restored the ovariectomy-induced alterations in bone weight, bone mineral content, bone strength, serum calcium, phosphorus and ALP, body weight and adipose weight nearly to normal levels. Furthermore, the SEM images of the femur bones of NR/CAL/06 (200 and 400 mg/kg, p.o.)-treated rats showed reduced pore formation and improved bone compactness compared with the OVX-control group.
These findings suggest that the methanolic extract of NR/CAL/06 possesses significant anti-osteoporotic activity and may be useful in the treatment of osteoporosis.
本研究旨在评估NR/CAL/06甲醇提取物对去卵巢（OVX）大鼠的抗骨质疏松活性。
在无菌条件下对雌性Sprague-Dawley大鼠进行双侧卵巢切除术，并将大鼠分为五组（n = 10）。评估了两种不同剂量的NR/CAL/06甲醇提取物（200和400 mg/kg，口服（p.o.））的抗骨质疏松活性，并使用雷洛昔芬（5.4 mg/kg，p.o.）作为参考标准。治疗持续90天。基于各种参数评估NR/CAL/06的抗骨质疏松潜力，这些参数包括体重、器官重量、骨重量、骨矿物质含量、骨强度、血清和尿液中的钙和磷以及血清碱性磷酸酶（ALP）。使用扫描电子显微镜（SEM）测量股骨的外径、长度和厚度。
大鼠双侧卵巢切除导致骨强度、骨矿物质含量和骨重量降低。SEM图像显示OVX大鼠的股骨结构多孔、穿孔且解体，骨长度和厚度减小。这些变化与血清钙、磷和ALP水平升高有关。还观察到体重和脂肪重量增加以及子宫重量减少，与假手术对照组相比，这些变化非常显著。用NR/CAL/（p.o.）甲醇提取物（200和400 mg/kg）治疗90天剂量依赖性地将卵巢切除引起的骨重量、骨矿物质含量、骨强度、血清钙、磷和ALP、体重和脂肪重量的改变恢复到接近正常水平。此外，与OVX对照组相比，经NR/CAL/06（200和400 mg/kg，p.o.）治疗的大鼠股骨的SEM图像显示孔隙形成减少且骨致密性改善。
这些发现表明NR/CAL/06甲醇提取物具有显著的抗骨质疏松活性，可能对骨质疏松症的治疗有用。
