Department of Neurosurgery, Neuro-oncology Laboratory, Affiliated Hospital of Jining Medical College, Jining, Shandong, 272029, PR China.
Brain Res. 2011 Jan 12;1368:82-90. doi: 10.1016/j.brainres.2010.10.051. Epub 2010 Oct 21.
This study tried to use CD133 positive U251 glioblastoma stem cells to establish nude mice model of transplanted tumor. CD133 positive U251 stem cells were isolated and identified. Twenty-five male nude mice were divided into three groups: U251 cell group, U251 stem cell group and normal control group. U251 cells and stem cells were respectively inoculated into mouse brain to establish model of transplanted tumor. Mice growing condition and behavior were observed. Magnetic resonance imaging (MRI) was performed to detect tumor growth in brain. HE staining assay, immunohistochemical assay and CD34 marked microvascular density (MVD) test were performed. As a result, the successful rates of both model groups were 100%. Growing condition and behavior in U251 stem cell group was more significantly exaggerated and tumor growth and image in magnetic resonance in U251 stem cell group was more significantly malignant than that in U251 cell group. It could be found that models in U251 stem cell group showed stronger pathogolical malignance features than that in U251 cell group. Glial fibrillary acidic protein (GFAP) expression in U251 cell and stem cell group showed the transplanted tumor originated from astrocytes. CD34 in U251 stem cell group was highest and significantly higher than that in the other two groups. In conclusion, use of U251 stem cells to establish transplanted tumor model in nude mice is an excellent method and the model is more likely and malignant than the one established by cancer cells, which showed a new animal model to research glioma.
本研究试图使用 CD133 阳性 U251 神经胶质瘤干细胞建立裸鼠移植瘤模型。分离并鉴定 CD133 阳性 U251 干细胞。将 25 只雄性裸鼠分为三组：U251 细胞组、U251 干细胞组和正常对照组。将 U251 细胞和干细胞分别接种到小鼠脑内，建立移植瘤模型。观察小鼠生长状况和行为表现。进行磁共振成像（MRI）检测脑内肿瘤生长情况。进行 HE 染色、免疫组织化学和 CD34 标记的微血管密度（MVD）检测。结果，两组模型的成功率均为 100%。U251 干细胞组的生长状况和行为表现更为明显，磁共振成像显示 U251 干细胞组的肿瘤生长和图像更为恶性。可以发现，U251 干细胞组模型表现出比 U251 细胞组更强的病理性恶性特征。U251 细胞和干细胞组的胶质纤维酸性蛋白（GFAP）表达显示移植瘤来源于星形胶质细胞。U251 干细胞组的 CD34 表达最高，明显高于其他两组。总之，使用 U251 干细胞建立裸鼠移植瘤模型是一种优良的方法，该模型比癌细胞建立的模型更具可能性和恶性，为研究神经胶质瘤提供了一种新的动物模型。
