Liu Yuwei, Yu Shupei, Zhang Yuanwei, Liu Xuan
Department of Electrical and Computer Engineering, New Jersey Institute of Technology, University Heights, Newark, New Jersey, 07105, USA.
Department of Cheminstry and Environmental Science, New Jersey Institute of Technology, University Heights, Newark, New Jersey, 07105, USA.
Biomed Opt Express. 2022 Dec 20;14(1):441-452. doi: 10.1364/BOE.479255. eCollection 2023 Jan 1.
The capability to image subtle mechanical motion at cellular and sub-cellular scales can be used to study how extracellular particles interact with cultured cells and, more generally, how cells interact with their environment. However, current technologies need to provide sufficient spatial resolution, temporal resolution, and motion sensitivity to image cellular and sub-cellular motion in the en face plane. To address this unmet need, we investigate a full-field Doppler phase microscopy (FF-DPM) technology based on an innovative optical computation strategy that enables depth-resolved imaging and phase quantification. In this study, we validated the motion tracking (displacements and velocities) capability of FF-DPM by imaging samples actuated by a piezo transducer (PZT). We demonstrated FF-DPM imaging of magnetic particles under different conditions with different motion characteristics. Our results show that free particles (suspended in a cell culture medium) had a significantly larger magnitude of motion than particles adhered to a cell. The key innovation of this study is the use of an optical computation strategy to perform depth-resolved phase quantification and Doppler measurement. The FF-DPM will have a significant impact, as it provides a unique capability to quantitatively measure subtle motion for models based on cultured cells.
在细胞和亚细胞尺度上对细微机械运动进行成像的能力，可用于研究细胞外颗粒如何与培养细胞相互作用，更广泛地说，用于研究细胞如何与它们的环境相互作用。然而，当前技术需要提供足够的空间分辨率、时间分辨率和运动灵敏度，以便在正面平面上对细胞和亚细胞运动进行成像。为满足这一未被满足的需求，我们研究了一种基于创新光学计算策略的全场多普勒相位显微镜（FF-DPM）技术，该策略能够实现深度分辨成像和相位量化。在本研究中，我们通过对由压电换能器（PZT）驱动的样本进行成像，验证了FF-DPM的运动跟踪（位移和速度）能力。我们展示了在不同条件下具有不同运动特征的磁性颗粒的FF-DPM成像。我们的结果表明，自由颗粒（悬浮在细胞培养基中）的运动幅度明显大于附着在细胞上的颗粒。本研究的关键创新在于使用光学计算策略进行深度分辨相位量化和多普勒测量。FF-DPM将产生重大影响，因为它为基于培养细胞的模型定量测量细微运动提供了独特能力。
