Han Xiaoxing, Brown Edward
Institute of Optics, University of Rochester, Rochester, New York 14627, USA.
Opt Express. 2010 May 10;18(10):10538-50. doi: 10.1364/OE.18.010538.
In this paper, we present a method to determine, for the first time, the ratio of forward-propagating second harmonic (SHG) signal to back-propagating SHG signal (F/B) in vivo on the surface of intact tissue samples without any biopsy or tissue sectioning, using only epidetection (i.e., via a single objective lens). This method has the additional benefit of using the confocal detection apparatus already contained within common commercially available two-photon laser-scanning microscopes, and hence can allow the measurement of the SHG F/B ratio in vivo with minimal purchase of new equipment.
在本文中，我们首次提出了一种方法，仅使用表皮检测（即通过单个物镜），在完整组织样本表面进行活体检测时，无需任何活检或组织切片，就能确定向前传播的二次谐波（SHG）信号与向后传播的SHG信号之比（F/B）。该方法还有一个额外的优点，即使用普通商用双光子激光扫描显微镜中已有的共聚焦检测装置，因此只需购买最少的新设备就能在活体中测量SHG F/B比。
