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一种带有内置分束器的多光子物镜设计，用于增强荧光收集。

A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection.

作者信息

McMullen Jesse D, Zipfel Warren R

机构信息

School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, USA.

出版信息

Opt Express. 2010 Mar 15;18(6):5390-8. doi: 10.1364/OE.18.005390.

DOI:10.1364/OE.18.005390
PMID:20389554
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3378349/
Abstract

We present a de novo design of an objective for use in multi-photon (MPM) and second harmonic generation (SHG) microscopy. This objective was designed to have a large field of view (FOV), while maintaining a moderate numerical aperture (NA) and relative straight forward construction. A dichroic beam splitter was incorporated within the objective itself allowing for an increase in the front aperture of the objective and corresponding enhancement of the solid angle of collected emission by an order of magnitude over existing designs.

摘要

我们展示了一种用于多光子显微镜（MPM）和二次谐波产生显微镜（SHG）的物镜的全新设计。该物镜设计为具有大视场（FOV），同时保持适度的数值孔径（NA）以及相对简单的结构。在物镜内部集成了一个二向色性分束器，这使得物镜的前孔径得以增大，并且收集发射光的立体角相较于现有设计提高了一个数量级。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e893/3378349/0eda43c4a48e/oe-18-6-5390-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e893/3378349/5f3e30d86c76/oe-18-6-5390-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e893/3378349/5d056bb0cd5a/oe-18-6-5390-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e893/3378349/a9952b9a9f67/oe-18-6-5390-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e893/3378349/0eda43c4a48e/oe-18-6-5390-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e893/3378349/5f3e30d86c76/oe-18-6-5390-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e893/3378349/5d056bb0cd5a/oe-18-6-5390-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e893/3378349/a9952b9a9f67/oe-18-6-5390-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e893/3378349/0eda43c4a48e/oe-18-6-5390-g004.jpg

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本文引用的文献

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Human bladder cancer diagnosis using Multiphoton microscopy.利用多光子显微镜进行人类膀胱癌诊断。
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