Kesler Gavriel, Shvero Dana Kesler, Tov Yariv Siman, Romanos George
Dental Laser Clinic, Department of Oral Rehabilitation, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel.
J Oral Implantol. 2011 Mar;37 Spec No:195-204. doi: 10.1563/AAID-JOI-D-09-00120.1. Epub 2010 Jun 16.
Er:YAG laser irradiation has been reported to enhance wound healing. However, no studies have evaluated the synthesis of growth factors after laser irradiation. The present study investigated the effects of laser irradiation on the amount of secretion of platelet derived growth factor (PDGF) in the wound, clarifying the effects of the Er:YAG laser on the bone healing. Osteotomies were prepared in the tibiae of 28 rats using an Er:YAG laser (test group). Maximum power of 8 watts, energy per pulse of 700 mJ, and frequency up to 50 Hz were used. The laser was used with external water irrigation, a spot size of 2 mm, energy per pulse of 500 to 1000 mJ/pulse, and energy density of 32 J/cm(2). Twenty eight additional rats served as a control group and their osteotomies were prepared with a drill 1.3 mm in diameter at 1000 rpm, with simultaneous saline irrigation. Two rats from the tested group and 2 from the control group were sacrificed on each day following surgery (1-14 days), and the tissue specimens were prepared for histologic evaluation. Immunohistochemical staining with anti-PDGF was performed after histologic examination. The difference between the PDGF staining intensities of the 2 treatment groups was analyzed using a multivariate logistic regression test. A significant rise in PDGF staining occurred in both groups 2-3 days following surgery. However, while high PDGF counts remained for the 2-week experimental period in the laser group, PDGF levels in the control group returned to baseline levels 8 days post surgery. The 2 groups (laser and control) were found to be different throughout the experiment, and the rat type was found to be a significant predictor (P = .000011). The present study demonstrated that Er:YAG laser irradiation seems to stimulate the secretion of PDGF in osteotomy sites in a rat model. It is possible that the high levels of PDGF are part of the mechanism that Er:YAG irradiation enhances and improves the healing of osteotomy sites.
据报道，铒激光照射可促进伤口愈合。然而，尚无研究评估激光照射后生长因子的合成情况。本研究调查了激光照射对伤口中血小板衍生生长因子（PDGF）分泌量的影响，以阐明铒激光对骨愈合的作用。使用铒激光在28只大鼠的胫骨上制备截骨术（试验组）。使用的最大功率为8瓦，每脉冲能量为700 mJ，频率高达50 Hz。激光使用外部水冲洗，光斑尺寸为2 mm，每脉冲能量为500至1000 mJ/脉冲，能量密度为32 J/cm²。另外28只大鼠作为对照组，用直径1.3 mm的钻头以1000 rpm的转速同时进行盐水冲洗来制备截骨术。术后每天（1 - 14天）处死试验组的2只大鼠和对照组的2只大鼠，并制备组织标本进行组织学评估。组织学检查后进行抗PDGF免疫组织化学染色。使用多因素逻辑回归检验分析两个治疗组PDGF染色强度的差异。术后2 - 3天，两组的PDGF染色均显著增加。然而，激光组在2周的实验期内PDGF计数一直保持较高水平，而对照组的PDGF水平在术后8天恢复到基线水平。发现两组（激光组和对照组）在整个实验过程中存在差异，并且大鼠类型是一个显著的预测因素（P = 0.000011）。本研究表明，铒激光照射似乎能刺激大鼠模型截骨部位PDGF的分泌。高水平的PDGF可能是铒激光照射增强和改善截骨部位愈合机制的一部分。
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