Gabrić Dragana, Blašković Marko, Gjorgijevska Elizabeta, Mladenov Mitko, Tašič Blaž, Jurič Ivona Bago, Ban Ticijana
Associate Professor, Department of Oral Surgery, School of Dental Medicine, University of Zagreb, Zagreb, Croatia.
PhD Student, School of Dental Medicine, University of Zagreb, Zagreb, Croatia.
J Oral Maxillofac Surg. 2016 Jan;74(1):18-28. doi: 10.1016/j.joms.2015.08.017. Epub 2015 Sep 21.
To analyze the healing of bone tissue treated with Er:YAG laser contact and noncontact modes of and piezosurgery in a rat model using triangular laser profilometry.
Twenty-four 10-week-old adult male Wistar rats were used in the study. Three osteotomies on the medial part of tibia were performed in each animal, 1 in the right tibia and 2 in the left tibia. The osteotomies were performed with a piezoelectric device set at maximal power and the Er:YAG laser in contact mode (power, 7.5 W; pulse energy, 375 mJ; repetition rate, 20 Hz; MSP mode) and noncontact mode (power, 7.5 W; pulse energy, 750 mJ; repetition rate, 10 Hz; QSP mode) with a novel type of circular, digitally controlled handpiece (x-Runner). After surgery, 6 animals were immediately euthanized (group 1), and the others were euthanized after 1 week (group 2, n = 6), 2 weeks (group 3, n = 6), and 3 weeks (group 4, n = 6). Bone healing after osteotomy was analyzed using a 3-dimensional laser scanning technique (ie, laser triangulation profilometry).
The volume reduction rates are similar for all 3 techniques (0.2 to 0.25 mm(3) per week). Greater volume reduction of 0.25 mm3 per week was observed for the Er:YAG laser in noncontact mode (x-Runner). After 3 weeks, almost complete healing of the prepared osteotomy was observed.
Within the limitations of this study, the osteotomies performed by the Er:YAG laser in digitally controlled noncontact mode healed the fastest.
使用三角激光轮廓测量法，在大鼠模型中分析经铒激光接触和非接触模式以及压电手术治疗的骨组织的愈合情况。
本研究使用了24只10周龄的成年雄性Wistar大鼠。每只动物在胫骨内侧进行三处截骨术，右侧胫骨1处，左侧胫骨2处。截骨术使用设置为最大功率的压电装置以及铒激光，分别采用接触模式（功率7.5W；脉冲能量375mJ；重复频率20Hz；MSP模式）和非接触模式（功率7.5W；脉冲能量750mJ；重复频率10Hz；QSP模式），使用新型圆形数控手持件（x-Runner）。手术后，立即对6只动物实施安乐死（第1组），其他动物在1周（第2组，n = 6）、2周（第3组，n = 6）和3周（第4组，n = 6）后实施安乐死。使用三维激光扫描技术（即激光三角测量轮廓术）分析截骨术后的骨愈合情况。
所有三种技术的体积减少率相似（每周0.2至0.25mm³）。在非接触模式（x-Runner）下使用铒激光观察到每周更大的体积减少量，为0.25mm³。3周后，观察到所制备的截骨术几乎完全愈合。
在本研究的局限性范围内，铒激光在数控非接触模式下进行的截骨术愈合最快。
