Instituto de Recursos Naturales y Agrobiología, CSIC, Apartado 1052, 41080 Sevilla, Spain.
Sci Total Environ. 2010 Aug 1;408(17):3711-5. doi: 10.1016/j.scitotenv.2010.05.008.
During the restoration of the church of "San Esteban" in Cuéllar (Segovia, Spain) a few sepulchres were opened. Among them was that of Doña Isabel de Zuazo, from the 16th century. Together with the corpse was found a series of printed documents from the 15th-16th centuries, most of which were bulls of indulgence. A microbiological study of the documents was carried out using techniques of isolation and molecular microbiology, together with scanning electron microscopy. Most of the identified bacteria were highly suggestive of a human origin, particularly the predominance of Clostridium species consistent with the flora of the human intestinal tract. Our results demonstrate that appreciable post-mortem migration of bacteria has taken place from the corpse to the historic documents. This can be explained considering that the documents were found on pelvic region, and were contaminated by body fluids and putrefaction.
在西班牙塞哥维亚的“圣埃斯特万”教堂修复期间，打开了几个坟墓。其中之一是 16 世纪的多娜·伊莎贝尔·德·祖阿佐的坟墓。与尸体一起发现的还有一系列来自 15 至 16 世纪的印刷文件，其中大部分是赎罪券。使用分离和分子微生物学技术以及扫描电子显微镜对文件进行了微生物学研究。鉴定出的大多数细菌高度提示来自人类，特别是与人类肠道菌群一致的梭状芽胞杆菌属的优势。我们的研究结果表明，大量细菌已经从尸体转移到历史文献中。考虑到这些文件是在骨盆区域发现的，并且被体液和腐烂物污染，因此可以解释这种情况。
