Florida Medical Entomology Laboratory, Department of Entomology and Nematology, University of Florida-IFAS, Vero Beach, FL, USA.
Am J Trop Med Hyg. 2010 Jul;83(1):126-34. doi: 10.4269/ajtmh.2010.09-0776.
Interactions between environmental and biological factors affect the vector competence of Culex pipiens quinquefasciatus for West Nile virus. Three age cohorts from two Cx. p. quinquefasciatus colonies were fed blood containing a low- or high-virus dose, and each group was held at two different extrinsic incubation temperatures (EIT) for 13 days. The colonies differed in the way that they responded to the effects of the environment on vector competence. The effects of mosquito age on aspects of vector competence were dependent on the EIT and dose, and they changed depending on the colony. Complex interactions must be considered in laboratory studies of vector competence, because the extent of the genetic and environmental variation controlling vector competence in nature is largely unknown. Differences in the environmental (EIT and dose) and biological (mosquito age and colony) effects from previous studies of Cx. p. quinquefasciatus vector competence for St. Louis encephalitis virus are discussed.
环境和生物因素的相互作用影响了库蚊对半番（Culex pipiens quinquefasciatus）对西尼罗河病毒的媒介效能。两个库蚊种群的三个龄期组分别喂食含低剂量或高剂量病毒的血液，并在两种不同的外潜伏期温度（EIT）下孵育 13 天。这两个种群对环境对媒介效能的影响的反应方式不同。蚊龄对媒介效能某些方面的影响取决于 EIT 和剂量，而且这种影响还因种群而异。在对媒介效能进行实验室研究时，必须考虑到复杂的相互作用，因为控制自然界中媒介效能的遗传和环境变异的程度在很大程度上是未知的。讨论了之前关于库蚊对半番（Culex pipiens quinquefasciatus）对圣路易斯脑炎病毒媒介效能的研究中环境（EIT 和剂量）和生物学（蚊龄和种群）效应的差异。
