Bakás L S, Disalvo E A
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.
Biochim Biophys Acta. 1991 Jun 18;1065(2):114-20. doi: 10.1016/0005-2736(91)90220-3.
In this paper, the influence of Ca2+ on the adsorption properties of 1,8-anilinonaphthalenesulfonate (ANS) and analogous probes to sonicated vesicles of phosphatidylcholine was studied by means of spectrofluorometry. The fluorescence of ANS added to the vesicle dispersion increases with the Ca2+ concentration in the inner media but remains constant when Ca2+ concentration is changed in the outside solution. However, the fluorescence decreases when large anions such as ClO4- are present in the external solution. Ca2+ inside large liposomes promotes a similar behaviour to that found in sonicated vesicles when they are osmotically contracted in hypertonic media. The results can be interpreted in terms of Ca2+ adsorption on the inner interface and a cooperative interaction between the monolayers.
本文通过荧光光谱法研究了Ca2+对1,8-苯胺基萘磺酸盐（ANS）及类似探针与超声处理的磷脂酰胆碱囊泡吸附特性的影响。添加到囊泡分散液中的ANS荧光随内部介质中Ca2+浓度的增加而增强，但当外部溶液中Ca2+浓度改变时荧光保持不变。然而，当外部溶液中存在诸如ClO4-等大阴离子时，荧光会降低。当大脂质体在高渗介质中发生渗透收缩时，其内部的Ca2+会引发与超声处理囊泡中类似的行为。这些结果可以通过Ca2+在内部界面的吸附以及单分子层之间的协同相互作用来解释。
