Bakás L S, Disalvo E A
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, UNLP, La Plata, Argentina.
Biochim Biophys Acta. 1989 Mar 13;979(3):352-60. doi: 10.1016/0005-2736(89)90256-3.
The incorporation of Ca2+ in the inner volume of egg phosphatidylcholine vesicles increases the fluorescence anisotropy of a diphenylhexatriene probe. This increase is higher than for Na+ at the same normality. An effect of the same magnitude is induced by Ca2+ when using binary lipid mixture (dioleoylphosphatidylcholine and dipalmitoylphosphatidylcholine) as long as the mixture is maintained below the phase-transition temperature of the saturated species. The influence of Ca2+ may be explained by an asymmetric distribution of the saturated and unsaturated lipids between the internal and the external monolayers.
钙离子掺入卵磷脂小泡内部会增加二苯基己三烯探针的荧光偏振。在相同当量浓度下，这种增加比钠离子引起的更高。当使用二元脂质混合物（二油酰磷脂酰胆碱和二棕榈酰磷脂酰胆碱）时，只要混合物保持在饱和物种的相变温度以下，钙离子就会产生相同程度的影响。钙离子的影响可以通过饱和脂质和不饱和脂质在内部和外部单分子层之间的不对称分布来解释。
