Institute of Pathology, University of Heidelberg, Heidelberg, Germany.
Int J Mol Med. 2010 Aug;26(2):281-8. doi: 10.3892/ijmm_00000463.
The protease ADAM10 influences progression and metastasis of cancer cells and is overexpressed in various malignancies. Therefore, the aim of our study was to evaluate the expression and potential function of ADAM10 in the pathophysiology of pancreatic cancer (PDAC). ADAM10 expression in normal pancreatic (NP), chronic pancreatitis (CP), PDAC tissues, as well as PDAC cell lines was determined. To evaluate whether rhADAM10 or ADAM10 silencing influences cancer cell viability, MTT assay was used. Matrigel invasion and wound healing assays were performed to observe influence on invasion and migration. ADAM10 mRNA was expressed in all samples of NP, CP and PDAC tissue and cell lines. Western blotting and immunohistochemistry revealed stronger ADAM10 expression in PDAC than in NP. ADAM10 silencing or rhADAM10 had no effect on cell viability. ADAM10 silencing markedly reduced invasiveness and migration of cancer cells. These findings establish ADAM10 as a contributing factor in PDAC invasion and metastasis.
蛋白酶 ADAM10 影响癌细胞的进展和转移，并且在各种恶性肿瘤中过度表达。因此，我们的研究目的是评估 ADAM10 在胰腺癌（PDAC）病理生理学中的表达和潜在功能。测定了正常胰腺（NP）、慢性胰腺炎（CP）、PDAC 组织以及 PDAC 细胞系中的 ADAM10 表达。为了评估 rhADAM10 或 ADAM10 沉默是否影响癌细胞活力，使用 MTT 测定法。进行 Matrigel 侵袭和划痕愈合测定以观察对侵袭和迁移的影响。ADAM10 mRNA 在 NP、CP 和 PDAC 组织和细胞系的所有样本中均有表达。Western blot 和免疫组化显示 PDAC 中的 ADAM10 表达强于 NP。ADAM10 沉默或 rhADAM10 对细胞活力没有影响。ADAM10 沉默显着降低了癌细胞的侵袭和迁移能力。这些发现确立了 ADAM10 是 PDAC 侵袭和转移的一个促成因素。
