解整合素金属蛋白酶10（ADAM10）在肝细胞癌中高表达，并与肿瘤进展相关。
A disintegrin and metalloprotease (ADAM)10 is highly expressed in hepatocellular carcinoma and is associated with tumour progression.
作者信息
Zhang Wei, Liu Songyang, Liu Kuai, Wang Yingchao, Ji Bai, Zhang Xuechun, Liu Yahui
机构信息
Department of Hepatopancreatobiliary Surgery, The First Hospital, Jilin University, Changchun, Jilin Province, China.
Department of Hepatopancreatobiliary Surgery, The First Hospital, Jilin University, Changchun, Jilin Province, China
出版信息
J Int Med Res. 2014 Jun;42(3):611-8. doi: 10.1177/0300060513505500. Epub 2014 Mar 26.
OBJECTIVE
A disintegrin and metalloprotease (ADAM)10 has been implicated in the progression of various solid tumours. Little is known, however, about its role in hepatocellular carcinoma (HCC). The aim of the present study was to evaluate the protein and transcript level expression of ADAM10 in HCC patients.
METHODS
Samples of HCC and adjacent noncancerous liver tissue were taken during liver resection surgery. Immunostaining was used to measure ADAM10 protein expression levels and quantitative reverse- transcription polymerase chain reaction was used to measure ADAM10 mRNA expression levels. Levels of ADAM10 were compared, and a survival analysis undertaken.
RESULTS
In total, 98 HCC patient samples were studied. There were significant associations between protein levels of ADAM10 and tumour grade, amount of tumour differentiation, tumour size and the presence of metastasis. Furthermore, ADAM10 protein expression was significantly associated with shortened patient survival.
CONCLUSIONS
ADAM10 is strongly expressed in a large proportion of HCC cases, which is in agreement with findings in other tumour entities. Expression of ADAM10 may serve as a useful molecular marker for HCC.
目的
解聚素和金属蛋白酶（ADAM）10与多种实体瘤的进展有关。然而，其在肝细胞癌（HCC）中的作用却鲜为人知。本研究的目的是评估ADAM10在HCC患者中的蛋白质和转录水平表达。
方法
在肝切除手术期间采集HCC和邻近非癌性肝组织样本。采用免疫染色法检测ADAM10蛋白表达水平，采用定量逆转录聚合酶链反应检测ADAM10 mRNA表达水平。比较ADAM10水平，并进行生存分析。
结果
共研究了98例HCC患者样本。ADAM10蛋白水平与肿瘤分级、肿瘤分化程度、肿瘤大小和转移情况之间存在显著关联。此外，ADAM10蛋白表达与患者生存期缩短显著相关。
结论
ADAM10在大部分HCC病例中高表达，这与其他肿瘤实体的研究结果一致。ADAM10的表达可能是HCC的一个有用分子标志物。
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