Yu Miao, Chen Quan, Li Qin, Teng Yunfei, Xiao Lin, Yang Guang, Ouyang Chenxi, Abdalla Ahmed Mohammed Elamin
Department of Vascular Surgery, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, China.
Department of Vascular Surgery, Gansu Provincial Hospital, Lanzhou 730000, China.
Iran J Public Health. 2022 Aug;51(8):1778-1789. doi: 10.18502/ijph.v51i8.10262.
Clinical investigations repurposing a disintegrin and metalloproteases 10 (ADAM10) as metastatic and thrombus marker have achieved encouraging results, but the mechanism behind this association remains unclear.
This study was carried out in NingXia and Wuhan, China from 2017 to 2021. The effects of ADAM10 expression on the metastatic and thrombus-associated genes: tissue factor (), P-selectin glycoprotein ligand-1 (), cathepsin G () and mucin 1 () were examined by immunofluorescence, qRTPCR and Western blotting analysis. Metastatic and thrombotic behaviors were evaluated using NODSCID mouse model.
The ADAM10 expression controlled the migration and invasion of pancreatic carcinoma cell-1(PANC-1), and significantly regulated the metastatic and thrombus-associated genes (<0.05). ADAM10 and MUC1 were regulated and aberrantly expressed by a dependent mechanism. Moreover, ADAM10 expression induced the progression of adenocarcinoma cells and thrombus formation in vivo.
Regulation of ADAM10 expression in cancer cells might effectively pave the way for a more potent anti-metastatic and anti-thrombotic approach and could regulate the invasion and migration of cancer cells.
将解整合素金属蛋白酶10（ADAM10）重新用作转移和血栓标志物的临床研究取得了令人鼓舞的结果，但这种关联背后的机制仍不清楚。
本研究于2017年至2021年在中国宁夏和武汉进行。通过免疫荧光、qRTPCR和蛋白质印迹分析检测ADAM10表达对转移和血栓相关基因：组织因子（）、P选择素糖蛋白配体-1（）、组织蛋白酶G（）和粘蛋白1（）的影响。使用NODSCID小鼠模型评估转移和血栓形成行为。
ADAM10表达控制胰腺癌细胞-1（PANC-1）的迁移和侵袭，并显著调节转移和血栓相关基因（<0.05）。ADAM10和MUC1通过依赖机制受到调节并异常表达。此外，ADAM10表达在体内诱导腺癌细胞进展和血栓形成。
调节癌细胞中ADAM10的表达可能有效地为更有效的抗转移和抗血栓方法铺平道路，并可调节癌细胞的侵袭和迁移。
