Department of Pharmacological and Pharmaceutical Science, College of Pharmacy, University of Houston, Houston, TX 77204-5037, USA.
Mol Cell Biochem. 2010 Nov;344(1-2):65-71. doi: 10.1007/s11010-010-0529-z. Epub 2010 Jul 2.
Earlier studies have reported an increase in the proximal tubule AT(2) receptor (AT(2)R) expression in diabetes, with a beneficial role in kidney function and blood pressure regulation. Here, we demonstrate that the increase in AT(2)R protein expression is associated with an increased expression of transcription factor IRF-1 in hyperglycemic rat and in high glucose-treated HK2 cells. Knock-down of IRF-1 by siRNA in HK2 cells prevented high glucose-induced AT(2)R up-regulation. The data suggest that IRF-1 is a transcriptional regulator of AT(2)R expression in hyperglycemia, and warrant further studies to understand the physiological role of IRF-1 along with AT(2)R in diabetic kidney.
早期研究报告称，糖尿病患者近端小管 AT(2) 受体 (AT(2)R) 的表达增加，这对肾功能和血压调节有有益作用。在这里，我们证明在高血糖大鼠和高葡萄糖处理的 HK2 细胞中，AT(2)R 蛋白表达的增加与转录因子 IRF-1 的表达增加有关。用 siRNA 在 HK2 细胞中敲低 IRF-1 可阻止高葡萄糖诱导的 AT(2)R 上调。数据表明，IRF-1 是高血糖中 AT(2)R 表达的转录调节剂，有必要进一步研究以了解 IRF-1 与 AT(2)R 在糖尿病肾脏中的生理作用。
