Korać Miloš, Rupnik Maja, Nikolić Nataša, Jovanović Milica, Tošić Tanja, Malinić Jovan, Mitrović Nikola, Marković Marko, Vujović Ankica, Peruničić Sanja, Bojović Ksenija, Djordjević Vladimir, Barać Aleksandra, Milošević Ivana
1University of Belgrade, Faculty of Medicine, Dr Subotića 8, 11000 Belgrade, Serbia.
2University Hospital for Infectious and Tropical Diseases, Clinical Centre of Serbia, Bulevar oslobođenja 16, 11000 Belgrade, Serbia.
Gut Pathog. 2020 May 22;12:26. doi: 10.1186/s13099-020-00364-7. eCollection 2020.
The global epidemic of nosocomial diarrhea caused by ( started in 2000, with high mortality rates and emergence of a new hypervirulent strain NAP1/BI/027. The aim of this study was to assess the presence of ribotype 027 and other ribotypes in a Serbian University Hospital, compare the temporal variability of ribotypes 3 years apart, as well as to compare clinical, demographic and laboratory characteristics and disease outcome among patients infected with 027 and non-027 ribotype. This was a prospective observational cohort study addressing 4-month intervals during 2014/2015 and 2017/2018.
Ribotyping was performed in 64 non-duplicate strains. Ribotype 027 was the most prevalent, and was detected in 53 (82.8%) patients (43/45 and 10/19 patients in 2014-2015 and 2017/2018, respectively). Other detected ribotypes were 001/072 in 4 (6.3%), 002 in 4 (6.3%), 014/020 in 2 (3.1%) and 176 in 1 (1.5%) patient. The percentage of the patients infected with ribotype 027 significantly decreased during the 3-year period, from 95.6 to 52.6% (< 0.001). Ribotype 027 infection was associated with fluoroquinolone treatment more frequently than infection with other ribotypes [33 (62.3%) vs. 2 (18.2%), = 0.010)]. A severe infection was diagnosed more often in patients with the detected ribotype 027 compared to those infected with non-027 ribotypes (p = 0.006). No significant difference in the mortality and recurrence rates was found between the patients infected with ribotype 027 and those infected with other ribotypes [10/53 (18.8%) vs. 2/11 (18.2%), p = 0.708, and 10/35 (28.6%) vs. 0/2 (0%), p = 1.000, respectively].
ribotype 027 was the most prevalent ribotype among patients in a large Serbian hospital, but there is a clear decreasing trend.
由[病原体名称未给出]引起的医院获得性腹泻全球流行始于2000年，死亡率高且出现了一种新的高毒力菌株NAP1/BI/027。本研究的目的是评估塞尔维亚一家大学医院中027核糖体分型及其他[病原体名称未给出]核糖体分型的存在情况，比较相隔3年的核糖体分型的时间变异性，以及比较感染027核糖体分型和非027核糖体分型患者的临床、人口统计学和实验室特征及疾病结局。这是一项前瞻性观察队列研究，涉及2014/2015年和2017/2018年期间每4个月的时间段。
对64株非重复的[病原体名称未给出]菌株进行了核糖体分型。027核糖体分型最为常见，在53例（82.8%）患者中检测到（2014 - 2015年和2017/2018年分别为43/45例和10/19例）。其他检测到的核糖体分型为4例（6.3%）的001/072、4例（6.3%）的002、2例（3.1%）的014/020和1例（1.5%）患者的176。在3年期间，感染027核糖体分型的患者百分比显著下降，从95.6%降至52.6%（<0.001）。027核糖体分型感染比其他核糖体分型感染更频繁地与氟喹诺酮治疗相关[33例（62.3%）对2例（18.2%），P = 0.010]。与感染非027核糖体分型的患者相比，检测到027核糖体分型的患者更常被诊断为严重[病原体名称未给出]感染（P = 0.006）。感染027核糖体分型的患者与感染其他核糖体分型的患者在死亡率和复发率方面未发现显著差异[分别为10/53（18.8%）对2/11（18.2%），P = 0.708，以及10/35（28.6%）对0/2（0%），P = 1.0]。
027核糖体分型是塞尔维亚一家大型医院患者中最常见的核糖体分型，但有明显的下降趋势。
