Veterinary Faculty, University of Ljubljana, Gerbiceva 60, SI-1000 Ljubljana, Slovenia.
Anaerobe. 2009 Dec;15(6):252-5. doi: 10.1016/j.anaerobe.2009.07.004. Epub 2009 Jul 24.
A study of Clostridium difficile diversity in pigs, calves and horses in Slovenia was conducted. A total of 547 samples were collected and C. difficile was isolated from 247/485 (50.9%) piglet samples, from 4/42 (9.5%) calf samples, and 1/20 (5%) horse samples. The isolates were characterized by toxinotyping, PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) using restriction endonuclease SmaI. Piglet isolates belonged to two toxinotypes (V and 0), four PCR-ribotypes (066, 029, SI 011, SI 010), and six pulsotypes. Bovine isolates were grouped into two toxinotypes (XIa and 0), three PCR-ribotypes (077, 002, 033), and three pulsotypes. The only equine isolate was indistinguishable from one calf isolate (XIa/033) in toxinotype, PCR-ribotype, and pulsotype. None of detected genotypes was present in all three animal hosts.
对斯洛文尼亚的猪、牛犊和马中的艰难梭菌多样性进行了研究。共采集了 547 份样本，从 247/485（50.9%）份仔猪样本、4/42（9.5%）份牛犊样本和 1/20（5%）份马样本中分离出艰难梭菌。通过毒素分型、PCR-核糖体分型和限制性内切酶 SmaI 脉冲场凝胶电泳（PFGE）对分离株进行了特征描述。仔猪分离株属于两个毒素型（V 和 0）、四个 PCR-核糖体型（066、029、SI 011、SI 010）和六个脉冲型。牛分离株分为两个毒素型（XIa 和 0）、三个 PCR-核糖体型（077、002、033）和三个脉冲型。唯一的马分离株在毒素型、PCR-核糖体型和脉冲型上与一个牛犊分离株（XIa/033）无法区分。在所有三种动物宿主中均未发现检测到的基因型。
