Nezhinskaia G I, Vladykin A L, Sapronov N S
Eksp Klin Farmakol. 2010 May;73(5):27-9.
The study was carried out on color guinea pigs, in which anaphylactic shock was induced by sensitizing and challenging doses of normal horse serum (HS). Administration of atropine, ipratropium, or ipratropium in combination with neostigmine led to reduction of sensitizing effect of HS: anaphylactic index was decreased up to (2.2 - 2.4) +/- 0.1 arbitrary units (a.u.). Methacine in combination with neostigmine (0.4 +/- 0.01 a.u.) or atropine in combination with galantamine (1.5 +/- 0.3 a.u.) effectively prevented the pathochemical stage of anaphylactic shock. Changes in plasma protein concentrations modified effects of methacine: application of methacine with IgG was protective (0.5 +/- 0.1 a.u.), while CRP abolish the antianaphylactic activity of methacine (3.8 +/- 0.1 a.u.). Guinea pigs which survived shock had a great number of AFC [(880 +/- 22) x 10(6) splenocytes in experimental group and (100 +/- 29) x 10(6) in control) and low concentration of 5-HT in the spleen (at a shock, 0.443 +/- 0.005; basal level, 1.532 +/- 0.004 ng/mg protein). Effective prevention of anaphylactic shock resulted in normalization of B cell activity and 5-HT level in the spleen. Thus, cholinergic drugs modulate the immunological and pathochemical stages of anaphylactic shock.
该研究在彩色豚鼠身上进行，通过致敏剂量和激发剂量的正常马血清（HS）诱导过敏性休克。给予阿托品、异丙托溴铵或异丙托溴铵与新斯的明联合使用可降低HS的致敏作用：过敏指数降低至（2.2 - 2.4）±0.1任意单位（a.u.）。美他环素与新斯的明联合使用（0.4±0.01 a.u.）或阿托品与加兰他敏联合使用（1.5±0.3 a.u.）可有效预防过敏性休克的病理化学阶段。血浆蛋白浓度的变化改变了美他环素的作用：美他环素与IgG联合使用具有保护作用（0.5±0.1 a.u.），而CRP则消除了美他环素的抗过敏活性（3.8±0.1 a.u.）。休克存活的豚鼠有大量的抗体形成细胞[实验组为（880±22）×10⁶脾细胞，对照组为（100±29）×10⁶]，脾脏中5 - HT浓度较低（休克时为0.443±0.005；基础水平为1.532±0.004 ng/mg蛋白质）。有效预防过敏性休克可使脾脏中B细胞活性和5 - HT水平恢复正常。因此，胆碱能药物可调节过敏性休克的免疫和病理化学阶段。
