齐墩果酸对过敏性休克的拮抗作用

[Antagonistic effect of oleanolic acid on anaphylactic shock].

作者信息

Zhang L R, Ma T X

机构信息

Department of Clinical Pharmacology, He-nan Medical University, Zhengzhou, China.

出版信息

Zhongguo Yao Li Xue Bao. 1995 Nov;16(6):527-30.

PMID:8732049

Abstract

AIM

To study the antagonistic effect of oleanolic acid (OA) on anaphylactic shock in guinea-pigs sensitized with 6-aminopenicillanic acid (APA)-protein.

METHODS

The anaphylactic shock was achieved by i.p. of APA-protein in guinea pigs. The serum antibody titers of guinea pigs were measured with the passive cutaneous anaphylaxis test, and the pulmonary histamine by the method of fluorometric determination. The degranulation of mast cells was examined by rat calvarian periosteum method.

RESULTS

In guinea pigs sensitized with APA-protein, OA 25, 50, 100 mg . kg-1 reduced anaphylactic shock rates from 100% (sensitized control group) to 70%, 18%, and 15% (OA pretreated group), and death rates from 100% to 70%, 18%, and 0%, respectively. OA had an inhibitory effect on the antibody formation and reduced the histamine contents in the lung tissues in the sensitized guinea pigs. In addition, the homologous passive cutaneous anaphylaxis in guinea pigs and the degranulation of mast cells of calvarial periosteum in rats were prevented by OA 25, 50, and 100 mg . kg-1.

CONCLUSION

The antagonistic effect of OA on anaphylactic shock was related to the inhibition of antibody formation and the reduction of histamine contents.

摘要

目的

研究齐墩果酸（OA）对6-氨基青霉烷酸（APA）-蛋白致敏豚鼠过敏性休克的拮抗作用。

方法

通过腹腔注射APA-蛋白使豚鼠发生过敏性休克。采用被动皮肤过敏试验测定豚鼠血清抗体效价，用荧光测定法测定肺组织组胺含量。采用大鼠颅骨骨膜法检测肥大细胞脱颗粒情况。

结果

在APA-蛋白致敏的豚鼠中，OA 25、50、100mg·kg-1使过敏性休克发生率从100%（致敏对照组）分别降至70%、18%和15%（OA预处理组），死亡率从100%分别降至70%、18%和0%。OA对致敏豚鼠抗体形成有抑制作用，并降低肺组织组胺含量。此外，25、50和100mg·kg-1的OA可抑制豚鼠的同种被动皮肤过敏反应及大鼠颅骨骨膜肥大细胞脱颗粒。