多方面表征氨纤维膨胀（AFEX）和稀酸预处理过程中形成的细胞壁分解产物。
Multifaceted characterization of cell wall decomposition products formed during ammonia fiber expansion (AFEX) and dilute acid based pretreatments.
机构信息
Biomass Conversion Research Laboratory, Chemical Engineering and Materials Science, Michigan State University, 3900 Collins Road, Suite 1045, Lansing, MI 48910, USA.
出版信息
Bioresour Technol. 2010 Nov;101(21):8429-38. doi: 10.1016/j.biortech.2010.06.027. Epub 2010 Jul 2.
Decomposition products formed/released during ammonia fiber expansion (AFEX) and dilute acid (DA) pretreatment of corn stover (CS) were quantified using robust mass spectrometry based analytical platforms. Ammonolytic cleavage of cell wall ester linkages during AFEX resulted in the formation of acetamide (25mg/g AFEX CS) and various phenolic amides (15mg/g AFEX CS) that are effective nutrients for downstream fermentation. After ammonolysis, Maillard reactions with carbonyl-containing intermediates represent the second largest sink for ammonia during AFEX. On the other hand, several carboxylic acids were formed (e.g. 35mg acetic acid/g DA CS) during DA pretreatment. Formation of furans was 36-fold lower for AFEX compared to DA treatment; while carboxylic acids (e.g. lactic and succinic acids) yield was 100-1000-fold lower during AFEX compared to previous reports using sodium hydroxide as pretreatment reagent.
在对玉米秸秆（CS）进行氨纤维膨胀（AFEX）和稀酸（DA）预处理的过程中形成/释放的分解产物，使用基于强大的质谱分析平台进行了定量分析。在 AFEX 过程中，细胞壁酯键的氨解导致了乙酰胺（25mg/g AFEX CS）和各种酚酰胺（15mg/g AFEX CS）的形成，它们是下游发酵的有效营养物质。氨解后，与含羰基中间体的美拉德反应是 AFEX 过程中氨的第二大消耗途径。另一方面，在 DA 预处理过程中形成了几种羧酸（例如 35mg/g DA CS 的乙酸）。与 DA 处理相比，AFEX 处理呋喃的形成量低 36 倍；而与之前使用氢氧化钠作为预处理试剂的报告相比，AFEX 过程中羧酸（例如乳酸和琥珀酸）的产率低 100-1000 倍。
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