Deconstruction Division, Joint BioEnergy Institute, Emeryville, CA, USA.
Bioresour Technol. 2011 Jul;102(13):6928-36. doi: 10.1016/j.biortech.2011.04.005. Epub 2011 Apr 8.
Ionic liquid (IL) and ammonia fiber expansion (AFEX) pretreatments were studied to develop the first direct side-by-side comparative assessment on their respective impacts on biomass structure, composition, process mass balance, and enzymatic saccharification efficiency. AFEX pretreatment completely preserves plant carbohydrates, whereas IL pretreatment extracts 76% of hemicellulose. In contrast to AFEX, the native crystal structure of the recovered corn stover from IL pretreatment was significantly disrupted. For both techniques, more than 70% of the theoretical sugar yield was attained after 48 h of hydrolysis using commercial enzyme cocktails. IL pretreatment requires less enzyme loading and a shorter hydrolysis time to reach 90% yields. Hemicellulase addition led to significant improvements in the yields of glucose and xylose for AFEX pretreated corn stover, but not for IL pretreated stover. These results provide new insights into the mechanisms of IL and AFEX pretreatment, as well as the advantages and disadvantages of each.
离子液体（IL）和氨纤维膨胀（AFEX）预处理进行了研究，以首次直接并排比较评估它们各自对生物质结构、组成、过程质量平衡和酶解效率的影响。AFEX 预处理完全保留了植物碳水化合物，而 IL 预处理提取了 76%的半纤维素。与 AFEX 相比，从 IL 预处理中回收的玉米秸秆的天然晶体结构明显被破坏。对于这两种技术，使用商业酶混合物进行 48 小时水解后，超过 70%的理论糖产率得以实现。IL 预处理需要更少的酶加载量和更短的水解时间即可达到 90%的产率。添加半纤维素酶可显著提高 AFEX 预处理玉米秸秆的葡萄糖和木糖得率，但对 IL 预处理的秸秆则不然。这些结果为 IL 和 AFEX 预处理的机制以及各自的优缺点提供了新的见解。
