School of Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei, Taiwan.
J Ethnopharmacol. 2011 Jul 14;136(3):511-7. doi: 10.1016/j.jep.2010.06.042. Epub 2010 Jul 3.
This study examined the modulating effects of Clematis chinensis Osbeck (Ranunculaeae) on pro-inflammatory and degradative mediators associated with inflammatory arthritis.
Primary human chondrocytes (PHC) were stimulated with IL-1β or lipopolysaccharide (LPS) to induce the enhanced release of prostaglandin E(2) (PGE(2)), metalloproteinase (MMP-3 and -13), and cyclooxygenase-2 (COX-2) protein expression. The (18)F-FDG microPET imaging system was used to evaluate the anti-arthritic effects of Clematis chinensisin vivo.
The acetone extracted Clematis chinensis (CC6) contained the most total saponins compared to other solvent's extracts and showed significant and dose-dependent inhibitory effects on PGE(2), MMP-3, -13, and COX-2 productions by LPS-stimulated PHC. Furthermore, CC6 also exerted inhibitory effects on 2-(18)F-fluoro-2-deoxy-d-glucose ((18)F-FDG) uptake when assessed by positron emission tomography (PET) uptake in the joints and serum PGE(2) of rabbits with knee joints injected with LPS.
The results suggest the significant chondroprotective effects of Clematis chinensis are through its anti-inflammatory and MMPs inhibitory abilities. Meanwhile, we established a new analysis method to evaluate the Chinese herbal anti-arthritic effects.
本研究探讨了威灵仙（毛茛科）对与炎症性关节炎相关的促炎和降解介质的调节作用。
原代人软骨细胞（PHC）用白细胞介素-1β（IL-1β）或脂多糖（LPS）刺激，以诱导前列腺素 E2（PGE2）、金属蛋白酶（MMP-3 和 -13）和环氧化酶-2（COX-2）蛋白表达的增强释放。使用 18F-FDG 微 PET 成像系统评估威灵仙体内的抗关节炎作用。
与其他溶剂提取物相比，威灵仙丙酮提取物（CC6）含有最多的总皂苷，对 LPS 刺激的 PHC 中 PGE2、MMP-3、-13 和 COX-2 的产生具有显著的、剂量依赖性的抑制作用。此外，CC6 还对兔膝关节注射 LPS 后关节和血清中 PGE2 的 2-（18）F-氟-2-脱氧-d-葡萄糖（18F-FDG）摄取产生抑制作用，通过正电子发射断层扫描（PET）摄取评估。
这些结果表明，威灵仙具有显著的软骨保护作用，其机制可能与其抗炎和 MMPs 抑制能力有关。同时，我们建立了一种新的分析方法来评估中药的抗关节炎作用。
