Max-Planck-Institut für biophysikalische Chemie, Abt. Spektroskopie, 37070 Göttingen, Germany.
Chem Phys Lipids. 2010 Sep;163(7):667-77. doi: 10.1016/j.chemphyslip.2010.06.005. Epub 2010 Jun 25.
Data on the molecular volumes of phospholipids and glycolipids in membranes are collected together in order to determine the contributions from the component groups, for as wide a range of lipids as possible, including sphingolipids. Wherever possible, the volumes of the methylene groups in the lipid chains are established from the dependence on chain length at fixed temperature in a given phase. In this way, it is also possible to determine the constant contribution from cis double bonds in the chains of monoenoic unsaturated phosphatidylcholines, and the volume of the branched methyl groups in isoacyl phosphatidylcholines. Issues concerning separation of contributions from the polar head groups from those of the chain terminal methyl groups are discussed. Molecular volumes of lipids in crystals are also analysed to provide information on head-group packing that can be compared with the situation in membranes, and used to set limits on the relative contributions from polar groups and terminal methyl groups. Comparisons are made with volumetric analyses based on diffraction studies of bilayers of single lipids. The parameters derived can be used to estimate molecular volumes of lipids for which dilatometric or densitometric data are lacking. Lipid volumes are determining parameters for lipid dynamics, membrane partitioning and permeation of solutes, and are essential quantities for the structural analysis of lipid membranes.
收集关于膜中磷脂和糖脂的分子体积的数据，以便确定尽可能广泛的脂质（包括鞘脂）的组成基团的贡献。在尽可能的情况下，从给定相在固定温度下的链长依赖性确定脂质链中亚甲基的体积。通过这种方式，还可以确定单烯不饱和磷脂酰胆碱链中顺式双键和异酰基磷脂酰胆碱中支化甲基的体积的恒定贡献。讨论了从极性头基团的贡献中分离链末端甲基基团的贡献的问题。还分析了晶体中脂质的分子体积，以提供有关头部基团堆积的信息，该信息可与膜中的情况进行比较，并用于限制极性基团和末端甲基基团的相对贡献。与基于单层脂质双层衍射研究的体积分析进行了比较。可以使用这些参数来估计缺乏膨胀或密度数据的脂质的分子体积。脂质体积是脂质动力学、膜分配和溶质渗透的决定参数，也是脂质膜结构分析的基本数量。
