Franceschini V, Ciani F
Department of Biology, Bologna University, Italy.
Cell Mol Biol. 1991;37(1):61-71.
Lectin histochemical studies were performed on paraffin embedded sections of the olfactory system of the eel to identify specific glycoconjugates on the surface of primary olfactory neurons. The olfactory receptors, the olfactory nerve fibres and their terminals in the bulbs were labelled with the lectins (SBA, BSA-I, BSA-I-B4 and DBA) HRP-conjugated or biotinylated. The lectin staining patterns indicate that the membrane of olfactory neurons of the eel had oligosaccharides with alpha-galactose and alpha-N-acetyl-D-galactosamine residues. These findings represent the demonstration of a molecular probe that recognizes specific sets of neurons. The identical histochemical features previously described in the olfactory neurons in amphibians suggest that these carbohydrate moieties might to related to modulation of the cell-cell interactions in the olfactory system of vertebrates.
对鳗鱼嗅觉系统的石蜡包埋切片进行凝集素组织化学研究，以识别初级嗅觉神经元表面的特定糖缀合物。嗅觉受体、嗅神经纤维及其在嗅球中的终末用凝集素（大豆凝集素、伴刀豆球蛋白A-I、伴刀豆球蛋白A-I-B4和荆豆凝集素）进行标记，这些凝集素与辣根过氧化物酶（HRP）结合或生物素化。凝集素染色模式表明，鳗鱼嗅觉神经元的膜含有带有α-半乳糖和α-N-乙酰-D-半乳糖胺残基的寡糖。这些发现代表了一种识别特定神经元群的分子探针的证明。先前在两栖动物嗅觉神经元中描述的相同组织化学特征表明，这些碳水化合物部分可能与脊椎动物嗅觉系统中细胞间相互作用的调节有关。
