Wilson K M, Siegal G, Lord E M
Cancer Center, University of Rochester Medical Center, New York 14642.
Cell Immunol. 1989 Oct 1;123(1):158-65. doi: 10.1016/0008-8749(89)90276-1.
We have directly demonstrated that macrophages present within solid EMT6 mammary tumors (of BALB/c origin) produce TNF-alpha (TNF). These tumor-associated macrophages lysed WEHI-164, a TNF-sensitive cell line, very efficiently. This cytotoxicity was abrogated in the presence of anti-TNF antisera. In contrast, EMT6 cells, the tumor from which the macrophages were obtained, were not effectively lysed by the macrophages and were 100-fold less sensitive to lysis by recombinant mouse TNF. Thus, marked heterogeneity exists among tumors regarding sensitivity to TNF-mediated cytotoxicity. Similarly, macrophages which infiltrate into EMT6 multicellular spheroids implanted into the peritoneal cavity as well as free cells within the cavity exhibited TNF-mediated cytotoxicity of WEHI-164 cells, but failed to lyse EMT6 cells. The kinetics of lysis by these cells was similar to that of recombinant mouse TNF.
我们已经直接证明，存在于实体EMT6乳腺肿瘤（起源于BALB/c）中的巨噬细胞会产生肿瘤坏死因子-α（TNF）。这些肿瘤相关巨噬细胞能非常有效地裂解对TNF敏感的细胞系WEHI-164。在抗TNF抗血清存在的情况下，这种细胞毒性被消除。相比之下，巨噬细胞所来源的肿瘤EMT6细胞不能被巨噬细胞有效裂解，并且对重组小鼠TNF裂解的敏感性低100倍。因此，肿瘤在对TNF介导的细胞毒性的敏感性方面存在明显的异质性。同样，浸润到植入腹膜腔的EMT6多细胞球体中的巨噬细胞以及腔内的游离细胞表现出对WEHI-164细胞的TNF介导的细胞毒性，但未能裂解EMT6细胞。这些细胞的裂解动力学与重组小鼠TNF的相似。
