Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
J Microbiol Methods. 2010 Sep;82(3):327-9. doi: 10.1016/j.mimet.2010.06.010. Epub 2010 Jun 30.
A new method for rapid enumeration of physiologically active mycobacteria was developed by acid-fast bacilli staining (Auramine O) following formazan reduction. Results can be obtained within 90 min by the optimized procedure, while more than one week is required for the widely used culture-dependent approach.
开发了一种新的快速计数方法，通过抗酸杆菌染色（金胺 O）后进行甲臜还原。通过优化程序，在 90 分钟内即可获得结果，而广泛使用的依赖培养的方法则需要一周以上的时间。
